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Rabbit polyclonal to DBNDD2
antigen sequence: VNVDTLEQVE LIDLGDPDAA DVFLPCEDPP PTPQSSGVDN HLEELSLPVP TSDRTTSRTS S, corresponding to amino acids 155-215 of Human DBNDD2 (UniProt Q9BQY9).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
May modulate the activity of casein kinase-1. Inhibits CSNK1D autophosphorylation (in vitro).
Detected in brain.
Belongs to the dysbindin family.
Information by UniProt
Casein kinase-1 binding protein antibody
Immunocytochemistry/ Immunofluorescence - Anti-DBNDD2 antibody (ab126348)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab126348 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-DBNDD2 antibody (ab126348)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBNDD2 antibody (ab126348)
ab126348, at a 1/1000 dilution, staining DBNDD2 in paraffin embedded Human cerebellum tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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