Overview

  • Product nameAnti-DBPA antibody
    See all DBPA primary antibodies
  • Description
    Rabbit polyclonal to DBPA
  • SpecificityThis antibody is specific for DBPA
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within synthetic peptide: LAAAAGSEDA EKKVLATKVL GTVKWFNVRN GYGFINRNDT KEDVFVHQTA, corresponding to amino acids 73-122 of Human DBPA

  • Positive control
    • Jurkat, HepG2 cell lysate and Fetal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab48952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/75000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-DBPA antibody images



  • Predicted band size : 40 kDa
    Western blot analysis of HeLa cell lysate labeling DBPA at 1.0µg/ml.
  • All lanes : Anti-DBPA antibody (ab48952) at 1 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Fetal muscle lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References for Anti-DBPA antibody (ab48952)

This product has been referenced in:
  • Wang W  et al. Antimicrobial peptide LL-37 promotes the proliferation and invasion of skin squamous cell carcinoma by upregulating DNA-binding protein A. Oncol Lett 12:1745-1752 (2016). IHC ; Human . Read more (PubMed: 27588122) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 30000 cells
Specification 293T
Gel Running Conditions Reduced Denaturing (4-20% TrisGlycine)
Blocking step 5% Milk, 1% BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Feb 12 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"