Overview

  • Product name
  • Description
    Rabbit polyclonal to DBR1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Xenopus tropicalis
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 68-324 of Human DBR1 (Uniprot Q9UK59).

  • Positive control
    • A549 whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 62 kDa.

Target

  • Function
    Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It may also participate in retrovirus replication via a RNA lariat intermediate in cDNA synthesis.
  • Sequence similarities
    Belongs to the lariat debranching enzyme family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DBR1 antibody
    • DBR1_HUMAN antibody
    • Debranching enzyme homolog 1 (S. cerevisiae) antibody
    • Lariat debranching enzyme antibody
    • RNA lariat debranching enzyme antibody
    see all

Images

  • All lanes : Anti-DBR1 antibody (ab154230) at 1/10000 dilution

    Lane 1 : Xenopus tropicalis oocyte 5 GV (nuclear) lysate
    Lane 2 : Xenopus tropicalis oocyte cytoplamsic lysate

    Lysates/proteins at 5 cells per lane.

    Secondary
    HRP-conjugated anti-rabbit polyclonal at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 62 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-DBR1 antibody (ab154230) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size : 62 kDa
    7.5% SDS PAGE

References

This product has been referenced in:
  • Talhouarne GJ & Gall JG Lariat intronic RNAs in the cytoplasm of Xenopus tropicalis oocytes. RNA 20:1476-87 (2014). Xenopus laevis, Xenopus tropicalis . Read more (PubMed: 25051970) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
5 cells
Gel Running Conditions
Reduced Denaturing (12.5%)
Sample
Xenopus tropicalis Cell lysate - nuclear (oocyte)
Specification
oocyte
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Feb 25 2014

Application
Western blot
Loading amount
10 cells
Gel Running Conditions
Reduced Denaturing (12.5%)
Sample
Xenopus laevis Cell lysate - nuclear (oocyte)
Specification
oocyte
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 26 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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