Overview

  • Product nameAnti-DBR1 antibody
    See all DBR1 primary antibodies
  • Description
    Mouse polyclonal to DBR1
  • SpecificityThis antibody is reactive against mammalian transfected lysate.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    DBR1 (NP_057300.2, 1 a.a. ~ 544 a.a) full-length human protein.

  • Positive control
    • DBR1 transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 62 kDa).

Target

  • FunctionCleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It may also participate in retrovirus replication via a RNA lariat intermediate in cDNA synthesis.
  • Sequence similaritiesBelongs to the lariat debranching enzyme family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DBR1 antibody
    • DBR1_HUMAN antibody
    • Debranching enzyme homolog 1 (S. cerevisiae) antibody
    • Lariat debranching enzyme antibody
    • RNA lariat debranching enzyme antibody
    see all

Anti-DBR1 antibody images

  • All lanes : Anti-DBR1 antibody (ab67891) at 1/500 dilution

    Lane 1 : DBR1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 62 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of purified antibody to DBR1 (ab67891) on HeLa cells. Antibody concentration was 10 ug/ml.

References for Anti-DBR1 antibody (ab67891)

ab67891 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 cells
Gel Running Conditions Reduced Denaturing (12.5%)
Sample Xenopus laevis Cell lysate - nuclear (oocyte)
Specification oocyte
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Feb 26 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"