Overview

  • Product nameAnti-DBT antibody
    See all DBT primary antibodies
  • Description
    Mouse polyclonal to DBT
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human) (AAH16675.1)

  • Positive control
    • Transfected 293T cell line Human liver tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab88471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa.

Target

  • RelevanceDBT or dihydrolipoamide branched chain transacylase E2 is a component of the BCKD (branched-chain alpha-keto dehydrogenase) complex which is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. The BCKD complex is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine.
  • Cellular localizationMitochondrion matrix.
  • Database links
  • Alternative names
    • BCATE2 antibody
    • BCKAD E2 subunit antibody
    • Branched chain acyltransferase, E2 component antibody
    • Dihydrolipoamide branched chain transacylase (E2 component of branched chain keto acid dehydrogenase complex; maple syrup urine disease) antibody
    • Dihydrolipoamide branched chain transacylase antibody
    • Dihydrolipoamide branched chain transacylase E2 antibody
    • Dihydrolipoyl transacylase antibody
    • Dihydrolipoyllysine residue (2 methylpropanoyl)transferase antibody
    • E2 antibody
    • E2 component of branched chain alpha keto acid dehydrogenase complex antibody
    • E2B antibody
    • Lipoamide acyltransferase component of mitochondrial branched chain alpha keto acid dehydrogenase complex antibody
    • Mitochondrial branched chain alpha keto acid dehydrogenase transacylase subunit (E2b) antibody
    see all

Anti-DBT antibody images

  • Anti-DBT antibody (ab88471) at 1 µg/ml + Human liver tissue lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 53 kDa
  • All lanes : Anti-DBT antibody (ab88471) at 1 µg/ml

    Lane 1 : DBT transfected 293T cell lysate
    Lane 2 : Untransfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Mouse IgG at 1/5000 dilution
    Lane 2 : Goat Anti-Mouse IgG at 1/5000 dilution


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References for Anti-DBT antibody (ab88471)

ab88471 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"