Overview

  • Product nameAnti-DBT antibody
    See all DBT primary antibodies
  • Description
    Rabbit polyclonal to DBT
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide designed within residues: YVCFFGYPSF KYSHPHHFLK TTAALRGQVV QFKLSDIGEG IREVTVKEWY, corresponding to amino acids 36 - 85 of Human DBT (NP_001909).

  • Positive control
    • HT1080 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceDBT or dihydrolipoamide branched chain transacylase E2 is a component of the BCKD (branched-chain alpha-keto dehydrogenase) complex which is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. The BCKD complex is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine.
  • Cellular localizationMitochondrion matrix.
  • Database links
  • Alternative names
    • BCATE2 antibody
    • BCKAD E2 subunit antibody
    • Branched chain acyltransferase, E2 component antibody
    • Dihydrolipoamide branched chain transacylase (E2 component of branched chain keto acid dehydrogenase complex; maple syrup urine disease) antibody
    • Dihydrolipoamide branched chain transacylase antibody
    • Dihydrolipoamide branched chain transacylase E2 antibody
    • Dihydrolipoyl transacylase antibody
    • Dihydrolipoyllysine residue (2 methylpropanoyl)transferase antibody
    • E2 antibody
    • E2 component of branched chain alpha keto acid dehydrogenase complex antibody
    • E2B antibody
    • Lipoamide acyltransferase component of mitochondrial branched chain alpha keto acid dehydrogenase complex antibody
    • Mitochondrial branched chain alpha keto acid dehydrogenase transacylase subunit (E2b) antibody
    see all

Anti-DBT antibody images

  • Anti-DBT antibody (ab90869) at 1 µg/ml + HT1080 cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 53 kDa
  • ab90869 staining DBT in kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab90869 staining DBT in skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab90869 staining DBT in spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-DBT antibody (ab90869)

ab90869 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab90869.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"