Overview

  • Product name
  • Description
    Rabbit polyclonal to DBT
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide designed within residues: YVCFFGYPSF KYSHPHHFLK TTAALRGQVV QFKLSDIGEG IREVTVKEWY, corresponding to amino acids 36 - 85 of Human DBT (NP_001909).

  • Positive control
    • HT1080 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    DBT or dihydrolipoamide branched chain transacylase E2 is a component of the BCKD (branched-chain alpha-keto dehydrogenase) complex which is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. The BCKD complex is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine.
  • Cellular localization
    Mitochondrion matrix.
  • Database links
  • Alternative names
    • BCATE2 antibody
    • BCKAD E2 subunit antibody
    • Branched chain acyltransferase, E2 component antibody
    • Dihydrolipoamide branched chain transacylase (E2 component of branched chain keto acid dehydrogenase complex; maple syrup urine disease) antibody
    • Dihydrolipoamide branched chain transacylase antibody
    • Dihydrolipoamide branched chain transacylase E2 antibody
    • Dihydrolipoyl transacylase antibody
    • Dihydrolipoyllysine residue (2 methylpropanoyl)transferase antibody
    • E2 antibody
    • E2 component of branched chain alpha keto acid dehydrogenase complex antibody
    • E2B antibody
    • Lipoamide acyltransferase component of mitochondrial branched chain alpha keto acid dehydrogenase complex antibody
    • Mitochondrial branched chain alpha keto acid dehydrogenase transacylase subunit (E2b) antibody
    see all

Images

  • Anti-DBT antibody (ab90869) at 1 µg/ml + HT1080 cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 53 kDa
  • ab90869 staining DBT in kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab90869 staining DBT in skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab90869 staining DBT in spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

ab90869 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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