Anti-DBX1 antibody (ab61488)


  • Product name
  • Description
    Rabbit polyclonal to DBX1
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse DBX1.

  • Positive control
    • This antibody gave a positive signal in the following mouse tissue lysates: E14 Embryo Brain; E14 Embryo Spinal Cord; E16 Embryo Brain; E16 Embryo Spinal Cord; E18 Embryo Brain; E18 Embryo Spinal Cord. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: NIH-3T3.



Our Abpromise guarantee covers the use of ab61488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).


  • Relevance
    Dbx1 homeodomain transcription factor is expressed in progenitors at the boundary between the dorsal and ventral plates of the caudal neural tube, from which postmitotic cells migrate tangentially to their final destination. Dbx1 is implicated in patterning the central nervous system during embryogenesis. Cell fate allocation and cell diversity are determined at very early stages in progenitor cells at precise coordinates along the dorsoventral and anteroposterior axis. In the spinal cord, the spatially restricted expression of Dbx1 in progenitors is critical in establishing interneuron cell fates and helps coordinate diverse phenotypic features. In the telencephalon, Dbx1 is expressed in restricted progenitor domains at the borders of the developing pallium.
  • Cellular localization
  • Database links
  • Alternative names
    • dbx antibody
    • Developing brain homeobox 1 antibody
    • Developing brain homeobox protein 1 antibody
    • Homeobox protein DBX1 antibody

Anti-DBX1 antibody images

  • All lanes : Anti-DBX1 antibody (ab61488) at 1 µg/ml

    Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
    Lane 2 : E14 Ms Embryo Spinal Cord Tissue Lysate
    Lane 3 : E16 Ms Embryo Brain Tissue Lysate
    Lane 4 : E16 Ms Embryo Spinal Cord Tissue Lysate
    Lane 5 : E18 Ms Embryo Brain Tissue Lysate
    Lane 6 : E18 Ms Embryo Spinal Cord Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • ICC/IF image of ab61488 stained NIH-3T3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab61488 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-DBX1 antibody (ab61488)

ab61488 has not yet been referenced specifically in any publications.

Product Wall

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Mouse Tissue sections (Brain)

Mr. Carl Hobbs

Verified customer

Submitted May 22 2014

Thank you for your reply.

I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to issue a credit note for you

Credit note ID: ######

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Thank you for taking the time to complete our questionnaire. The details you have kindly provided will provide us with vital information for our monitoring of product quality.

I appreciate the time you have spent in the laboratory and unders...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the r...

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Thank you for your reply. Frozen sections are falling in our category of immunohistochemistry (IHC-Fr). Please keep this in mind when submitting your Abreview.
Mouse samples have been tested, but human, rat and cow samples are only predicted to re...

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Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Zebra finch Tissue sections (embryonal spinal cord)
embryonal spinal cord
Yes - 0.1% TX100 in PBS
Blocking step
Roti-Immunoblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Dr. Robert Storm

Verified customer

Submitted Feb 06 2012


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