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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-dbx2 antibody
See all dbx2 products (2) ...
Description
Rabbit polyclonal to dbx2
Specificity
We are unable to source appropriate lysates to test this antibody inhouse; testing feedback is invited on ab33170.
Tested applications
WB, IHC-Frmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat, Chicken
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Mouse dbx2.
(Peptide available as ab27732.)
Positive control
expected positive is mouse embryonic spinal cord e10.5 (Neuron, Vol 29, 367-384, February 2001)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Intracellular
Stem Cells >> Neural Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
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Immunohistochemistry (Frozen sections) - dbx2 antibody (ab25554)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab25554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. (PubMed: 20409825)
IHC-Fr: 1/1000 - 1/4000.
Target
Relevance
Members of the Dbx family are implicated in regionalization of the CNS. Dbx2 is restricted to the ventricular region of the embryonic CNS as well as some of the mesenchymal cells, such as limb buds and tooth germs. BMP regulation of the expression boundaries of the homeobox proteins Dbx2, Pax6 and Msx1 generates precursor populations with distinct developmental potentials.
Cellular localization
Nuclear
Alternative names
- Dbx 2 antibody
- developing brain homeobox 2 antibody
- FLJ16139 antibody
- hlx3 antibody
Database links
- Entrez Gene: 223843 Mouse
- Entrez Gene: 541457 Rat
- SwissProt: Q8BZD0 Mouse
- Unigene: 152937 Mouse
Anti-dbx2 antibody images:
Immunohistochemistry (Frozen sections) - dbx2 antibody (ab25554)
Immunohistochemistical detection of dbx2 protein on mouse embryonic spinal cord (E11.5) using Rabbit polyclonal to dbx2 (ab25554) at 1/4000. 1% BSA was used as a blocking agent (for 10 mins). Sections were fixed with paraformaldehyde, no antigen retrieval step was necessary. This image shows the expected spatially restricted dbx2 expression in intermediate neural progenitors of the spinal cord.
J Briscoe, Natl Inst Med Res, United Kingdom
References for Anti-dbx2 antibody (ab25554)
This product has been referenced in:
- Norman RXet al. Tubby-like protein 3 (TULP3) regulates patterning in the mouse embryo through inhibition of Hedgehog signaling. Hum Mol Genet 18:1740-54 (2009). IHC-Fr; Mouse.Read more (PubMed: 19286674) »
- Ko HWet al. Analysis of hedgehog signaling in mouse intraflagellar transport mutants. Methods Cell Biol 93:347-69 (2009). WB; Mouse.Read more (PubMed: 20409825) »
See all 2 publications for this product
Publishing research using ab25554? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"