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Immunohistochemistry (Frozen sections) - dbx2 antibody (ab25554)
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Product Name
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dbx2 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to dbx2
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Mouse dbx2.
(Peptide available as ab27732.)
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Reacts with
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Ms
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Predicted to react
(species key)
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Rat
(Due to sequence homology)
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Specificity
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We are unable to source appropriate lysates to test this antibody inhouse; testing feedback is invited on ab33170.
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Tested applications
(see key)
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IHC-Fr
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-Fr: 1/1000 - 1/4000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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expected positive is mouse embryonic spinal cord e10.5 (Neuron, Vol 29, 367-384, February 2001)
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Cellular localization
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Nuclear
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Research areas
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Stem Cells >> Mesenchymal Stem Cells >> Intracellular
Stem Cells >> Neural Stem Cells >> Intracellular
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
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Relevance
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Members of the Dbx family are implicated in regionalization of the CNS. Dbx2 is restricted to the ventricular region of the embryonic CNS as well as some of the mesenchymal cells, such as limb buds and tooth germs. BMP regulation of the expression boundaries of the homeobox proteins Dbx2, Pax6 and Msx1 generates precursor populations with distinct developmental potentials.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.900 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this dbx2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for dbx2 antibody images, references, products related to ab25554 and other tools.
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dbx2 antibody images:
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 Immunohistochemistry (Frozen sections) - dbx2 antibody (ab25554)
Immunohistochemistical detection of dbx2 protein on mouse embryonic spinal cord (E11.5) using Rabbit polyclonal to dbx2 (ab25554) at 1/4000. 1% BSA was used as a blocking agent (for 10 mins). Sections were fixed with paraformaldehyde, no antigen retrieval step was necessary. This image shows the expected spatially restricted dbx2 expression in intermediate neural progenitors of the spinal cord. J Briscoe, Natl Inst Med Res, United Kingdom
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References for dbx2 antibody (ab25554)
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This product has been used in:
Norman RX et al. Tubby-like protein 3 (TULP3) regulates patterning in the mouse embryo through inhibition of Hedgehog signaling. Hum Mol Genet 18:1740-54 (2009). IHC-Fr; Mouse. PubMed: 19286674
If you publish research using ab25554 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to dbx2
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dbx2 antibody - more information
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Customer reviews (feedback) regarding dbx2 antibody |
Customer FAQs regarding dbx2 antibody |
Protocols for dbx2 antibody |
Price and availability of products related to dbx2 antibody |
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dbx2 antibody - related products:
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dbx2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about dbx2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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