The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceMembers of the Dbx family are implicated in regionalization of the CNS. Dbx2 is restricted to the ventricular region of the embryonic CNS as well as some of the mesenchymal cells, such as limb buds and tooth germs. BMP regulation of the expression boundaries of the homeobox proteins Dbx2, Pax6 and Msx1 generates precursor populations with distinct developmental potentials.