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Tissue/ cell preparation (NIH/3T3 transfectants stably expressing human DC-SIGN1).
Our Abpromise guarantee covers the use of ab13487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Blocking||Use at an assay dependent concentration. PubMed: 23424649|
|IP||Use at an assay dependent concentration. PubMed: 20630938|
|Flow Cyt||Use 25-50µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|Inhibition Assay||Use a concentration of 5 µg/ml. This antibody shows inhibition of DC-SIGN expressing NIH-3T3 cell adhesion to ICAM-3 coated 96 well plates.|