Overview

  • Product nameAnti-DC-SIGN antibody [120507]
    See all DC-SIGN primary antibodies
  • Description
    Mouse monoclonal [120507] to DC-SIGN
  • SpecificityThe antibody does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.
  • Tested applicationsSuitable for: Blocking, IP, Flow Cyt, Inhibition Assaymore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (NIH/3T3 transfectants stably expressing human DC-SIGN1).

  • Positive control
    • Transfected NIH/3T3 cells and monocyte derived dendritic cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab13487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration. PubMed: 23424649
IP Use at an assay dependent concentration. PubMed: 20630938
Flow Cyt Use 25-50µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Inhibition Assay Use a concentration of 5 µg/ml. This antibody shows inhibition of DC-SIGN expressing NIH-3T3 cell adhesion to ICAM-3 coated 96 well plates.

Target

  • FunctionPathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
    On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.
  • Tissue specificityPredominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • DomainThe tandem repeat domain, also called neck domain, mediates oligomerization.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C type lectin domain family 4 member L antibody
    • C-type lectin domain family 4 member L antibody
    • CD 209 antibody
    • CD209 antibody
    • CD209 antigen antibody
    • CD209 antigen-like protein A antibody
    • CD209 molecule antibody
    • CD209_HUMAN antibody
    • Cd209a antibody
    • CDSIGN antibody
    • CIRE antibody
    • CLEC4L antibody
    • DC SIGN antibody
    • DC SIGN1 antibody
    • DC-SIGN antibody
    • DC-SIGN1 antibody
    • DCSIGN antibody
    • Dendritic cell specific ICAM 3 grabbing nonintegrin 1 antibody
    • Dendritic cell specific ICAM3 grabbing nonintegrin 1 antibody
    • Dendritic cell-specific ICAM-3-grabbing non-integrin 1 antibody
    • Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin antibody
    • Dengue fever, protection against, included antibody
    • Dentritic Cell Specific ICAM3 Grabbing Nonintegrin antibody
    • HIV GP120 Binding Protein antibody
    • MGC129965 antibody
    • MGC130443 antibody
    • SIGN-R1 antibody
    • SIGNR5 antibody
    see all

References for Anti-DC-SIGN antibody [120507] (ab13487)

This product has been referenced in:
  • Hillaire ML  et al. Binding of DC-SIGN to the hemagglutinin of influenza A viruses supports virus replication in DC-SIGN expressing cells. PLoS One 8:e56164 (2013). Blocking . Read more (PubMed: 23424649) »
  • Costantini C  et al. On the potential involvement of CD11d in co-stimulating the production of interferon-? by natural killer cells upon interaction with neutrophils via intercellular adhesion molecule-3. Haematologica 96:1543-7 (2011). Human . Read more (PubMed: 21712539) »

See all 9 Publications for this product

Product Wall

Thank you for your inquiry. Regarding ab13487, we expect this product to be stable for up to 5 years when stored at -20C. I have checked our available stock and we do have some antibody from this production batch with the lot numbers GR24878-2 and ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"