Overview

  • Product nameAnti-DC-SIGN antibody [5D7]
    See all DC-SIGN primary antibodies
  • Description
    Mouse monoclonal [5D7] to DC-SIGN
  • Tested applicationsSuitable for: WB, IHC-P, IHC-FoFr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Rhesus monkey
  • Immunogen

    A recombinant protein fragment corresponding to the extracellular region of human DC-SIGN.

  • Positive control
    • WB: Human uterus tissue lysate. IHC: lymph node tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab59192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 46 kDa).
IHC-P Use at an assay dependent concentration.
IHC-FoFr Use a concentration of 5 - 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 23734186

Target

  • FunctionPathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
    On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.
  • Tissue specificityPredominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • DomainThe tandem repeat domain, also called neck domain, mediates oligomerization.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C type lectin domain family 4 member L antibody
    • C-type lectin domain family 4 member L antibody
    • CD 209 antibody
    • CD209 antibody
    • CD209 antigen antibody
    • CD209 antigen-like protein A antibody
    • CD209 molecule antibody
    • CD209_HUMAN antibody
    • Cd209a antibody
    • CDSIGN antibody
    • CIRE antibody
    • CLEC4L antibody
    • DC SIGN antibody
    • DC SIGN1 antibody
    • DC-SIGN antibody
    • DC-SIGN1 antibody
    • DCSIGN antibody
    • Dendritic cell specific ICAM 3 grabbing nonintegrin 1 antibody
    • Dendritic cell specific ICAM3 grabbing nonintegrin 1 antibody
    • Dendritic cell-specific ICAM-3-grabbing non-integrin 1 antibody
    • Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin antibody
    • Dengue fever, protection against, included antibody
    • Dentritic Cell Specific ICAM3 Grabbing Nonintegrin antibody
    • HIV GP120 Binding Protein antibody
    • MGC129965 antibody
    • MGC130443 antibody
    • SIGN-R1 antibody
    • SIGNR5 antibody
    see all

Anti-DC-SIGN antibody [5D7] images

  • Lane 1 : Anti-DC-SIGN antibody [5D7] (ab59192) at 0.25 µg/ml
    Lane 2 : Anti-DC-SIGN antibody [5D7] (ab59192) at 0.5 µg/ml

    Lane 1 : human uterus tissue lysate
    Lane 2 : human uterus tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat-anti mouse

    Predicted band size : 46 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
  • ab59192 at 5ug/ml staining DC-SIGN in lymph node tissue by Immunohistochemistry.

References for Anti-DC-SIGN antibody [5D7] (ab59192)

This product has been referenced in:
  • Herrera M  et al. Cancer-associated fibroblast and M2 macrophage markers together predict outcome in colorectal cancer patients. Cancer Sci 104:437-44 (2013). Human . Read more (PubMed: 23298232) »
  • Tsai YM  et al. Functional interaction of cockroach allergens and mannose receptor (CD206) in human circulating fibrocytes. PLoS One 8:e64105 (2013). Flow Cyt ; Human . Read more (PubMed: 23734186) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (Lymph Node)
Specification Lymph Node
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 37°C
Username

Mr. Peter Sullivan

Verified customer

Submitted Aug 13 2012

Thank you for your call today and for letting us know about the trouble with these antibodies. As we discussed, I'm sending a free of charge vial of each antibody on the order ***, which should arrive tomorrow. Please keep me updated about the performa...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. I attached a copy of your general IHC-P protocol to this email which can also be found using this link: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"