Overview

  • Product nameAnti-DC-SIGN antibody [5H10]
    See all DC-SIGN primary antibodies
  • Description
    Rat monoclonal [5H10] to DC-SIGN
  • Specificityab95634 does not cross react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4.
  • Tested applicationsSuitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    A peptide derived from the extracellular region of Mouse DC-SIGN.

  • Positive control
    • DC-SIGN transfected CHO cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab95634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.

Target

  • FunctionPathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
    On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.
  • Tissue specificityPredominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • DomainThe tandem repeat domain, also called neck domain, mediates oligomerization.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C type lectin domain family 4 member L antibody
    • C-type lectin domain family 4 member L antibody
    • CD 209 antibody
    • CD209 antibody
    • CD209 antigen antibody
    • CD209 antigen-like protein A antibody
    • CD209 molecule antibody
    • CD209_HUMAN antibody
    • Cd209a antibody
    • CDSIGN antibody
    • CIRE antibody
    • CLEC4L antibody
    • DC SIGN antibody
    • DC SIGN1 antibody
    • DC-SIGN antibody
    • DC-SIGN1 antibody
    • DCSIGN antibody
    • Dendritic cell specific ICAM 3 grabbing nonintegrin 1 antibody
    • Dendritic cell specific ICAM3 grabbing nonintegrin 1 antibody
    • Dendritic cell-specific ICAM-3-grabbing non-integrin 1 antibody
    • Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin antibody
    • Dengue fever, protection against, included antibody
    • Dentritic Cell Specific ICAM3 Grabbing Nonintegrin antibody
    • HIV GP120 Binding Protein antibody
    • MGC129965 antibody
    • MGC130443 antibody
    • SIGN-R1 antibody
    • SIGNR5 antibody
    see all

Anti-DC-SIGN antibody [5H10] images

  • ab95634 staining DC-SIGN in Mouse macrophage cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% Serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 2% FBS) for 1 hour at 25°C. An Alexa Fluor®488-conjugated Donkey anti-rat IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-DC-SIGN antibody [5H10] (ab95634) at 1 µg/ml

    Lane 1 : DC-SIGN transfected CHO cell lysate loaded at 1 x 105 cells
    Lane 2 : Untransfected control CHO cell lysates loaded at 1 x 105 cells

    Secondary
    anti-Rat IgG HRP

    Predicted band size : 27 kDa

References for Anti-DC-SIGN antibody [5H10] (ab95634)

This product has been referenced in:
  • Caminschi I  et al. Functional comparison of mouse CIRE/mouse DC-SIGN and human DC-SIGN. Int Immunol 18:741-53 (2006). Read more (PubMed: 16569675) »
  • O'Keeffe M  et al. Mouse plasmacytoid cells: long-lived cells, heterogeneous in surface phenotype and function, that differentiate into CD8(+) dendritic cells only after microbial stimulus. J Exp Med 196:1307-19 (2002). Flow Cyt ; Mouse . Read more (PubMed: 12438422) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (macrophage)
Specification macrophage
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Apr 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"