Overview

  • Product nameAnti-DCAF13 antibody
    See all DCAF13 primary antibodies
  • Description
    Rabbit polyclonal to DCAF13
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Hamster, Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster
  • Immunogen

    Synthetic peptide: WSPAGRATEM KVKMLSRNPD NYVRETKLDL QRVPRNYDPA LHPFEVPREY , corresponding to a region within N terminal amino acids 144 - 193 of Human DCAF13 (NP_056235)

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionPossible role in ribosomal RNA processing (By similarity). May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the WD repeat DCAF13/WDSOF1 family.
    Contains 7 WD repeats.
  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DCA13_HUMAN antibody
    • DCAF13 antibody
    • DDB1 and CUL4 associated factor 13 antibody
    • DDB1- and CUL4-associated factor 13 antibody
    • DKFZP564O0463 antibody
    • GM83 antibody
    • HSPC064 antibody
    • MGC126859 antibody
    • MGC138247 antibody
    • WD repeat and SOF domain-containing protein 1 antibody
    • WD repeats and SOF1 domain containing antibody
    • WDSOF1 antibody
    see all

Anti-DCAF13 antibody images

  • Anti-DCAF13 antibody (ab83546) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)

References for Anti-DCAF13 antibody (ab83546)

ab83546 has not yet been referenced specifically in any publications.

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