Recombinant
RabMAb

Anti-DCAF13 antibody [EPR18723] (ab195121)

Overview

  • Product name
    Anti-DCAF13 antibody [EPR18723]
    See all DCAF13 primary antibodies
  • Description
    Rabbit monoclonal [EPR18723] to DCAF13
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse DCAF13 aa 1-100. The exact sequence is proprietary.
    Database link: Q6PAC3

  • Positive control
    • WB: C6, PC-12, NIH/3T3, Neuro-2a, F9, HeLa and 293T whole cell lysates; Mouse kidney and spleen lysates; Rat spleen lysate; Human fetal liver, fetal heart and fetal kidney lysates. IHC-P: Mouse ovary and colon tissues. IP: NIH/3T3 whole cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab195121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/50.

Target

  • Function
    Possible role in ribosomal RNA processing (By similarity). May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the WD repeat DCAF13/WDSOF1 family.
    Contains 7 WD repeats.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DCA13_HUMAN antibody
    • DCAF13 antibody
    • DDB1 and CUL4 associated factor 13 antibody
    • DDB1- and CUL4-associated factor 13 antibody
    • DKFZP564O0463 antibody
    • GM83 antibody
    • HSPC064 antibody
    • MGC126859 antibody
    • MGC138247 antibody
    • WD repeat and SOF domain-containing protein 1 antibody
    • WD repeats and SOF1 domain containing antibody
    • WDSOF1 antibody
    see all

Images

  • All lanes : Anti-DCAF13 antibody [EPR18723] (ab195121) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 4 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
    Lane 5 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 51 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-DCAF13 antibody [EPR18723] (ab195121) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 51 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-DCAF13 antibody [EPR18723] (ab195121) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : 293T (Human epithelial cell line from embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 51 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-DCAF13 antibody [EPR18723] (ab195121) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size : 51 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded mouse ovary tissue labeling DCAF13 with ab195121 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Mainly nuclear staining on mouse ovary is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP).

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling DCAF13 with ab195121 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Nuclear/nucleolus staining with additional cytoplasm staining on mouse colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP).

  • DCAF13 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab195121 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab195121 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.

    Lane 1: NIH/3T3 whole cell lysate, 10µg (Input).

    Lane 2: ab195121 IP in NIH/3T3 whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal - Isotype Control (ab172730) instead of ab195121 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

ab195121 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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