Overview

  • Product nameAnti-DCAMKL1 antibody
    See all DCAMKL1 primary antibodies
  • Description
    Rabbit polyclonal to DCAMKL1
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human DCAMKL1 aa 690-720 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab110799)

  • Positive control
    • WB: Mouse heart and human brain tissue lysates. IHC-P: Human hepatocarcinoma tissue. ICC/IF: MCF7 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • Purification notesThis antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt 1/33. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ELISA 1/100.
WB 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IHC-P 1/10 - 1/50.

Target

  • FunctionProbable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
  • Tissue specificityIn fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 2 doublecortin domains.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium/calmodulin-dependent protein kinase type I-like CPG16 antibody
    • CL1 antibody
    • CLICK1 antibody
    • Cpg16 antibody
    • DCDC3A antibody
    • Dcl antibody
    • Dclk antibody
    • Dclk1 antibody
    • DCLK1_HUMAN antibody
    • Doublecortin domain-containing protein 3A antibody
    • Doublecortin-like and CAM kinase-like 1 antibody
    • Doublecortin-like kinase 1 antibody
    • KIAA0369 antibody
    • Serine/threonine-protein kinase DCAMKL1 antibody
    • Serine/threonine-protein kinase DCLK1 antibody
    see all

Anti-DCAMKL1 antibody images

  • Anti-DCAMKL1 antibody (ab37994) at 1/750 dilution (for 20 hours at 4°C) + Human brain whole tissue lysate at 22 µg

    Secondary
    An undiluted HRP-conjugated Goat anti-rabbit polyclonal
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 82 kDa
    Observed band size : 82 kDa


    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview

    Blocking step: 5% Milk for 1 hour at 18C°C

    See Abreview

  • Anti-DCAMKL1 antibody (ab37994) at 1/1000 dilution + Mouse heart tissue lysate at 35 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling DCAMKL1 with ab37994. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling DCAMKL1 with ab37994. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.

  • ICC/IF image of ab37994 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37994, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-DCAMKL1 antibody (ab37994)

This product has been referenced in:
  • Neradugomma NK  et al. Prolactin signaling enhances colon cancer stemness by modulating Notch signaling in a Jak2-STAT3/ERK manner. Carcinogenesis N/A:N/A (2013). Read more (PubMed: 24265293) »
  • Gonzalez LM  et al. Cell lineage identification and stem cell culture in a porcine model for the study of intestinal epithelial regeneration. PLoS One 8:e66465 (2013). Read more (PubMed: 23840480) »

See all 9 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH6
Sample Mouse Tissue sections (Intestine)
Specification Intestine
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 16 2014

Thank you for your phone call.

A suitable isotype control for ab31704 and ab37994 would be: ab27478

http://www.abcam.com/Rabbit-polyclonal-IgG-ab27478.html


Please find herethe discount code and more detailed informat...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab31704.

To check the status of the order please contact o...

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Thanks for your email and for the detailed description of the problem.

You have done a good job in attempts to optimize the protocol. This item is purchased relativelyfrequently and there has only been 1 other recent complaint (within the las...

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Thank you for taking the time to complete our questionnaire and sending me the publication. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibodies.

The details you have kindly provided will enable us ...

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Thank you for contacting us. We know that the antibody recognizes the 2 isoforms: AL, BL (which corresponds to isoforms 2 and 4) due to sequence homology. I hope this information is helpful to you. Please do not hesitate to contact us if you need...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Intestine)
Specification Human Intestine
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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Verified customer

Submitted Sep 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Human Brain)
Loading amount 22 µg
Specification Human Brain
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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Verified customer

Submitted Aug 20 2010

Application ELISA
Sample Rat Cell (Enterocytes)
Specification Enterocytes
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Type Sandwich (Capture)
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Submitted Jun 03 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (mouse liver)
Specification mouse liver
Preparation Cell harvesting/tissue preparation method: from mouse
Sample buffer: PBS containing 10% serum
Fixation Formaldehyde
Permeabilization No
Username

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Verified customer

Submitted May 12 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"