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Products:Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Overview
Product name
Anti-DCAMKL1 antibody
See all DCAMKL1 products (6) ...
Description
Rabbit polyclonal to DCAMKL1
Tested applications
ICC/IF, Flow Cyt, ELISA, WB, IHC-P, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH, corresponding to C terminal amino acids selected from the sequence of human DCAMKL1 within aa 700-800.
Positive control
Mouse heart tissue lysate, Human hepatocarcinoma
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
Flow Cyt: 1/33
ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).Can be blocked with DCAMKL1 peptide (ab110799).
IHC-P: 1/50 - 1/100.
IHC-P: Use at an assay dependent concentration.
Target
Function
Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
Tissue specificity
In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 2 doublecortin domains.
Contains 1 protein kinase domain.
Target information above from: UniProt accessionO15075
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Calcium/calmodulin-dependent protein kinase type I-like CPG16 antibody
- Cpg16 antibody
- Dcl antibody
see all
Database links
- Entrez Gene: 9201 Human
- Entrez Gene: 13175 Mouse
- Omim: 604742 Human
- SwissProt: O15075 Human
- SwissProt: Q9JLM8 Mouse
- Unigene: 507755 Human
- Unigene: 295263 Mouse
- Unigene: 393242 Mouse
see all
Anti-DCAMKL1 antibody images:
Western blot - DCAMLK1 antibody (ab37994)
Anti-DCAMKL1 antibody (ab37994) + mouse heart tissue lysate
Predicted band size : 82 kDa
Observed band size : 82 kDa
Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - DCAMLK1 antibody (ab37994)
Ab37994 at (conc) staining human hepatocarcinoma; formalin fixed, paraffin embedded. Ab37994 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Western blot - DCAMKL1 antibody (ab37994)
Anti-DCAMKL1 antibody (ab37994) at 1/750 dilution (for 20 hours at 4°C) + Human brain whole tissue lysate at 22 µg
Secondary
An undiluted HRP-conjugated Goat anti-rabbit polyclonal
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 82 kDa
Exposure time : 3 minutes
Blocking step: 5% Milk for 1 hour at 18C°C
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence-DCAMKL1 antibody(ab37994)
ICC/IF image of ab37994 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37994, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-DCAMKL1 antibody (ab37994)
This product has been referenced in:
- Gerbe Fet al. Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium. J Cell Biol 192:767-80 (2011).Read more (PubMed: 21383077) »
- von Furstenberg RJet al. Sorting mouse jejunal epithelial cells with CD24 yields a population with characteristics of intestinal stem cells. Am J Physiol Gastrointest Liver Physiol 300:G409-17 (2011).Read more (PubMed: 21183658) »
See all 6 publications for this product
Publishing research using ab37994? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"