Overview

  • Product name
  • Description
    Rabbit polyclonal to DCAMKL1
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human DCAMKL1 aa 690-720 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab110799)

  • Positive control
    • WB: Mouse heart and human brain tissue lysates. IHC-P: Human hepatocarcinoma tissue. ICC/IF: MCF7 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt 1/33.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ELISA 1/100.
WB 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IHC-P 1/10 - 1/50.

Target

  • Function
    Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
  • Tissue specificity
    In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 2 doublecortin domains.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium/calmodulin-dependent protein kinase type I-like CPG16 antibody
    • CL1 antibody
    • CLICK1 antibody
    • Cpg16 antibody
    • DCDC3A antibody
    • Dcl antibody
    • Dclk antibody
    • Dclk1 antibody
    • DCLK1_HUMAN antibody
    • Doublecortin domain-containing protein 3A antibody
    • Doublecortin-like and CAM kinase-like 1 antibody
    • Doublecortin-like kinase 1 antibody
    • KIAA0369 antibody
    • Serine/threonine-protein kinase DCAMKL1 antibody
    • Serine/threonine-protein kinase DCLK1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded normal mouse small intestine tissue labeling DCAMKL1 with ab37994 at 1/10 dilution

  • Anti-DCAMKL1 antibody (ab37994) at 1/1000 dilution + Mouse heart tissue lysate at 35 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • ICC/IF image of ab37994 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37994, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-DCAMKL1 antibody (ab37994) at 1/750 dilution (for 20 hours at 4°C) + Human brain whole tissue lysate at 22 µg

    Secondary
    An undiluted HRP-conjugated Goat anti-rabbit polyclonal
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 82 kDa
    Observed band size : 82 kDa


    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview

    Blocking step: 5% Milk for 1 hour at 18C°C

    See Abreview

  • All lanes : Anti-DCAMKL1 antibody (ab37994) at 1/8000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate
    Lane 4 : TT whole lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 82 kDa

References

This product has been referenced in:
  • Nakagawa A  et al. Selective and reversible suppression of intestinal stem cell differentiation by pharmacological inhibition of BET bromodomains. Sci Rep 6:20390 (2016). IHC-P ; Mouse . Read more (PubMed: 26856877) »
  • Terry NA  et al. Impaired enteroendocrine development in intestinal-specific Islet1 mouse mutants causes impaired glucose homeostasis. Am J Physiol Gastrointest Liver Physiol 307:G979-91 (2014). Read more (PubMed: 25214396) »

See all 12 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH6
Sample
Mouse Tissue sections (Intestine)
Specification
Intestine
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 16 2014

Thank you for your phone call.

A suitable isotype control for ab31704 and ab37994 would be: ab27478

http://www.abcam.com/Rabbit-polyclonal-IgG-ab27478.html


Please find herethe discount code and more detailed informat...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab31704.

To check the status of the order please contact o...

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Thanks for your email and for the detailed description of the problem.

You have done a good job in attempts to optimize the protocol. This item is purchased relativelyfrequently and there has only been 1 other recent complaint (within the las...

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Thank you for taking the time to complete our questionnaire and sending me the publication. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibodies.

The details you have kindly provided will enable us ...

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Thank you for contacting us. We know that the antibody recognizes the 2 isoforms: AL, BL (which corresponds to isoforms 2 and 4) due to sequence homology. I hope this information is helpful to you. Please do not hesitate to contact us if you need...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Intestine)
Specification
Human Intestine
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Abcam user community

Verified customer

Submitted Sep 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Human Brain)
Loading amount
22 µg
Specification
Human Brain
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Abcam user community

Verified customer

Submitted Aug 20 2010

Application
ELISA
Sample
Rat Cell (Enterocytes)
Specification
Enterocytes
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Type
Sandwich (Capture)
Username

Abcam user community

Verified customer

Submitted Jun 03 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (mouse liver)
Specification
mouse liver
Preparation
Cell harvesting/tissue preparation method: from mouse
Sample buffer: PBS containing 10% serum
Fixation
Formaldehyde
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted May 12 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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