Validated using a knockout cell line
Recombinant
RabMAb

Anti-DCAMKL1 antibody [EPR6085] (ab109029)

Overview

  • Product name
    Anti-DCAMKL1 antibody [EPR6085]
    See all DCAMKL1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6085] to DCAMKL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DCAMKL1 aa 650-750. The exact sequence is proprietary.

  • Positive control
    • Human fetal lung, NIH3T3, and SH-SY5Y lysates, Human brain tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50-82 kDa (predicted molecular weight: 82 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
    • Tissue specificity
      In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
      Contains 2 doublecortin domains.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Calcium/calmodulin-dependent protein kinase type I-like CPG16 antibody
      • CL1 antibody
      • CLICK1 antibody
      • Cpg16 antibody
      • DCDC3A antibody
      • Dcl antibody
      • Dclk antibody
      • Dclk1 antibody
      • DCLK1_HUMAN antibody
      • Doublecortin domain-containing protein 3A antibody
      • Doublecortin-like and CAM kinase-like 1 antibody
      • Doublecortin-like kinase 1 antibody
      • KIAA0369 antibody
      • Serine/threonine-protein kinase DCAMKL1 antibody
      • Serine/threonine-protein kinase DCLK1 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Knockout HAP1 whole cell lysate (20 µg)
      Lane 3: DCLK1  (KO) whole cell lysate (20 µg)
      Lane 4: Empty whole cell lysate (20 µg)
      Lane 5: Hu brain whole cell lysate (20 µg)
      Lanes 1 - 5: Merged signal (red and green). Green - ab109029 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109029 was shown to specifically react with DCLK1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when knockout samples were used. Wild-type and knockout samples were subjected to SDS-PAGE. Ab109029 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.


    • All lanes : Anti-DCAMKL1 antibody [EPR6085] (ab109029) at 1/1000 dilution

      Lane 1 : Human fetal lung lysate
      Lane 2 : NIH3T3 lysate
      Lane 3 : SH-SY5Y lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 82 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109029 at a dilution of 1/100.

    References

    This product has been referenced in:
    • Fan M  et al. Expression and prognostic significance of doublecortin-like kinase 1 in patients with hepatocellular carcinoma. Oncol Lett 14:7529-7537 (2017). Read more (PubMed: 29344199) »
    • Feng Y  et al. Sox9 induction, ectopic Paneth cells, and mitotic spindle axis defects in mouse colon adenomatous epithelium arising from conditional biallelic Apc inactivation. Am J Pathol 183:493-503 (2013). ICC/IF ; Mouse . Read more (PubMed: 23769888) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Miss. Narin Ilgım Ardıç

    Verified customer

    Submitted May 23 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
    Permeabilization
    No
    Specification
    lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Apr 17 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Apr 17 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (stomach)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
    Permeabilization
    No
    Specification
    stomach
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Apr 17 2018

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% Normal buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Apr 10 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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