The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 84 kDa.
FunctionProtein kinase with a significantly reduced Ca2+/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 2 doublecortin domains. Contains 1 protein kinase domain.
DomainThe doublecortin domains are involved in the colocalization with microtubules.
Cellular localizationCytoplasm > cytoskeleton. Colocalizes with microtubules.