Anti-DCC antibody (ab125280)


  • Product nameAnti-DCC antibody
    See all DCC primary antibodies
  • Description
    Rabbit polyclonal to DCC
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 1380-1447 of Human DCC.

  • Positive control
    • Human brain tissue.


Associated products


Our Abpromise guarantee covers the use of ab125280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 160-190 kDa (predicted molecular weight: 158 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P Use a concentration of 0.5 - 2 µg/ml.


  • FunctionReceptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene.
  • Tissue specificityFound in axons of the central and peripheral nervous system and in differentiated cell types of the intestine. Not expressed in colorectal tumor cells that lost their capacity to differentiate into mucus producing cells.
  • Involvement in diseaseDefects in DCC are the cause of congenital mirror movements (MIMOC) [MIM:157600]. Mirror movements are contralateral involuntary movements that mirror voluntary ones. While mirror movements are occasionally found in young children, persistence beyond the age of 10 is abnormal. Mirror movements occur more commonly in the upper extremities.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. DCC family.
    Contains 6 fibronectin type-III domains.
    Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Colorectal cancer related chromosome sequence 18 antibody
    • Colorectal cancer suppressor antibody
    • CRC 18 antibody
    • CRC18 antibody
    • CRCR 1 antibody
    • CRCR1 antibody
    • DCC antibody
    • DCC_HUMAN antibody
    • Deleted in colorectal cancer protein antibody
    • Deleted in colorectal carcinoma antibody
    • Immunoglobulin superfamily DCC subclass member 1 antibody
    • Netrin receptor DCC antibody
    • Tumor suppressor protein DCC antibody
    see all

References for Anti-DCC antibody (ab125280)

ab125280 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Dorsal Root Ganglia)
Permeabilization Yes - PBS + 0,2% Triton X-100
Specification Dorsal Root Ganglia
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 13 2016

Ab125280 has been tested with human brain tissue samples, so ab29466 is a good positive control to use with this antibody. According to UniProt, DCC is expressed in axons in the central nervous system, so the human brain tissue should definit...

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