DCC antibody [AF5] (ab16793)

Product Name 

DCC antibody [AF5]

Product type 

Primary antibodies

Description 

Mouse monoclonal [AF5] to DCC

Immunogen 

Immunogen used was the extracellular domain of the human DCC protein. DCC, p190, has been identified as the netrin-1 receptor.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ICC, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC (IF): Use at a concentration of 1 - 5 µg/ml.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 158 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Membrane; single pass type I membrane protein.

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Cell Biology >> Apoptosis >> Receptors >> Associated Proteins

Relevance 

Deleted in colorectal cancer (DCC, chromosome 18q21) was originally identified as a putative tumor suppressor gene that is lost in more than 70% of colorectal cancers. The gene has also been deleted in several other types of cancer. The DCC protein is a type I transmembrane glycoprotein that belongs to the immunoglobulin (Ig) superfamily. The extracellular domain is composed of four Ig like domains and six fibronectin type III repeats. Native DCC is found in three isoforms. Two forms, a long and a short isoform, are produced from the same gene but have different initiation sites. The third isoform, produced by alternative splicing, is expressed only in embryonic tissue. Mouse DCC extracellular domain shares 97% and 99% homology with human and rat DCC extracellular domains, respectively. In adults, DCC is highly expressed in the brain but is also expressed at very low levels in multiple normal tissues. In the embyro, high levels of expression are detected in the brain and neural tube. DCC functions as a receptor or a component of a receptor for netrins and mediates the effects of netrins on commissural axons. Netrins are chemoattractants responsible for the guidance of commissural axons at the midline and of motor axons to the their target muscles. DCC induces apoptosis in the absence of ligand binding, blocks apoptosis when engaged by netrin 1, and also acts as a caspase substrate.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

AF5

Isotype 

IgG1

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 0.25% Gelatin, 0.05M Sodium phosphate

Material safety datasheets (MSDS) for this product:
Sodium Azide MSDS
Sodium Phosphate MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

References for DCC antibody [AF5] (ab16793)

  This product has been used in:

Mai J et al. Axon initiation and growth cone turning on bound protein gradients. J Neurosci 29:7450-8 (2009). PubMed: 19515913

If you publish research using ab16793 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding DCC antibody [AF5]

Customer FAQs regarding DCC antibody [AF5]

Protocols for DCC antibody [AF5]

Price and availability of products related to DCC antibody [AF5]

DCC antibody [AF5] - related products: