![Flow Cytometry - Anti-DCC antibody [AF5] (ab16793) image](#DCC-Primary-antibodies-ab16793-1.jpg)
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Products:Cell Biology >> Apoptosis >> Receptors >> Associated Proteins
Overview
Product name
Anti-DCC antibody [AF5]
See all DCC products (7) ...
Description
Mouse monoclonal [AF5] to DCC
Tested applications
IHC-P, Flow Cyt, ICC, WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Immunogen used was the extracellular domain of the human DCC protein. DCC, p190, has been identified as the netrin-1 receptor.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 0.25% Gelatin, 0.05M Sodium phosphate
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
AF5
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Cell Biology >> Apoptosis >> Receptors >> Associated Proteins
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Flow Cytometry - Anti-DCC antibody [AF5] (ab16793)
![Flow Cytometry - Anti-DCC antibody [AF5] (ab16793) image](/ps/datasheet/images/16/ab16793/DCC-Primary-antibodies-ab16793-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab16793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. (PubMed: 21215373)
Flow Cyt: Use 1µg for 106 cells.
ICC: Use a concentration of 1 - 5 µg/ml.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 158 kDa.
IP: Use at an assay dependent dilution.
Target
Function
Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene.
Tissue specificity
Found in axons of the central and peripheral nervous system and in differentiated cell types of the intestine. Not expressed in colorectal tumor cells that lost their capacity to differentiate into mucus producing cells.
Involvement in disease
Defects in DCC are the cause of congenital mirror movements (MIMOC) [MIM:157600]. Mirror movements are contralateral involuntary movements that mirror voluntary ones. While mirror movements are occasionally found in young children, persistence beyond the age of 10 is abnormal. Mirror movements occur more commonly in the upper extremities.
Sequence similarities
Belongs to the immunoglobulin superfamily. DCC family.
Contains 6 fibronectin type-III domains.
Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
Post-translational
modifications
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
Cellular localization
Membrane.
Target information above from: UniProt accessionP43146
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Colorectal cancer related chromosome sequence 18 antibody
- Colorectal cancer suppressor antibody
- CRC 18 antibody
see all
Database links
- Entrez Gene: 1630 Human
- Entrez Gene: 13176 Mouse
- Omim: 120470 Human
- SwissProt: P43146 Human
- SwissProt: P70211 Mouse
- Unigene: 162025 Human
- Unigene: 167882 Mouse
Anti-DCC antibody [AF5] images:
Flow Cytometry - Anti-DCC antibody [AF5] (ab16793)
Overlay histogram showing UG-87 cells stained with ab16793 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16793, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
References for Anti-DCC antibody [AF5] (ab16793)
This product has been referenced in:
- Bai Get al. Presenilin-dependent receptor processing is required for axon guidance. Cell 144:106-18 (2011). IHC-P, IP; Mouse.Read more (PubMed: 21215373) »
- Mai Jet al. Axon initiation and growth cone turning on bound protein gradients. J Neurosci 29:7450-8 (2009).Read more (PubMed: 19515913) »
See all 2 publications for this product
Publishing research using ab16793? Please let us know so that we can cite the reference in this datasheet
![DCC antibody [AF5] for WB in Xenopus laevis (16793)](/ps/Reviews/Images/ab12677_70677.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"