Recombinant
RabMAb

Anti-DCDC2 antibody [EPR11126(B)] (ab157186)

Overview

  • Product name
    Anti-DCDC2 antibody [EPR11126(B)]
    See all DCDC2 primary antibodies
  • Description
    Rabbit monoclonal [EPR11126(B)] to DCDC2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human DCDC2 (UniProt Q9UHG0).

  • Positive control
    • Fetal brain, HeLa and 293T lysates; Human kidney and pancreas tissues; HeLa cells; 293T cells; Immunoprecipitation pellet from fetal brain lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
IHC-P 1/250 - 1/500.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    May be involved in neuronal migration during development of the cerebral neocortex.
  • Tissue specificity
    Ubiquitously expressed. In brain, highly expressed in the entorhinal cortex, inferior temporal cortex, medial temporal cortex, hypothalamus, amygdala and hippocampus.
  • Involvement in disease
    Defects in DCDC2 may be a cause of susceptibility to dyslexia type 2 (DYX2) [MIM:600202]; also known as specific reading disability type 2. Dyslexia is a relatively common, complex cognitive disorder that affects 5% to 10% of school-aged children. The disorder is characterized by an impairment of reading performance despite adequate motivational, educational and intellectual opportunities and in the absence of sensory or neurological disability.
  • Sequence similarities
    Contains 2 doublecortin domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • DCDC 2 antibody
    • DCDC2 antibody
    • DCDC2_HUMAN antibody
    • DCDC2A antibody
    • Doublecortin domain containing 2 antibody
    • doublecortin domain containing protein 2 antibody
    • Doublecortin domain-containing protein 2 antibody
    • HGNC:18141 antibody
    • KIAA1154 antibody
    • Protein RU2S antibody
    • RU 2 antibody
    • RU2 antibody
    • RU2S antibody
    • RU2S protein antibody
    see all

Images

  • All lanes : Anti-DCDC2 antibody [EPR11126(B)] (ab157186) at 1/1000 dilution

    Lanes 1 & 4 : Fetal brain lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 52 kDa

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCDC2 with ab157186 at 1/250 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling DCDC2 with ab157186 at 1/250 dilution.
  • Immunofluorescent analysis of HeLa cells labeling DCDC2 with ab157186 at 1/250 dilution.
  • Flow cytometric analysis of permeabilized 293T cells labeling DCDC2 with ab157186 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
  • Anti-DCDC2 antibody [EPR11126(B)] (ab157186) at 1/1000 dilution + immunoprecipitation pellet from fetal brain lysate

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 52 kDa

References

This product has been referenced in:
  • Tammimies K  et al. Ciliary dyslexia candidate genes DYX1C1 and DCDC2 are regulated by Regulatory Factor X (RFX) transcription factors through X-box promoter motifs. FASEB J 30:3578-3587 (2016). ICC/IF ; Human . Read more (PubMed: 27451412) »
  • Schueler M  et al. DCDC2 mutations cause a renal-hepatic ciliopathy by disrupting Wnt signaling. Am J Hum Genet 96:81-92 (2015). IF . Read more (PubMed: 25557784) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
IHC - Wholemount
Sample
Rat Tissue (Inner ear)
Specification
Inner ear
Username

Dr. M'Hamed Grati

Verified customer

Submitted Feb 04 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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