Overview

  • Product nameAnti-DCK antibody [16E12]
    See all DCK primary antibodies
  • Description
    Mouse monoclonal [16E12] to DCK
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human DCK (NP_000779) produced in HEK293T cells.

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY DCK. HeLa cells. Jurkat cells.
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Applications

Our Abpromise guarantee covers the use of ab118994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 31 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionRequired for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
  • Sequence similaritiesBelongs to the DCK/DGK family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • dCK antibody
    • DCK protein antibody
    • DCK_HUMAN antibody
    • Deoxycytidine kinase antibody
    • Deoxynucleoside kinase antibody
    • EC 2.7.1 antibody
    • EC 2.7.1.74 antibody
    • MGC117410 antibody
    • MGC138632 antibody
    • OTTHUMP00000219118 antibody
    • OTTHUMP00000219119 antibody
    see all

Anti-DCK antibody [16E12] images

  • All lanes : Anti-DCK antibody [16E12] (ab118994) at 1/2000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY DCK cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 31 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210767)
  • HEK293T cells transfected with either DCK overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab118994 at 1/100, and then analyzed by flow cytometry.
  • Flow cytometric analysis of HeLa cells, using ab118994 at 1/100 (Red) compared to a nonspecific negative control antibody (Blue).
  • Flow cytometric analysis of Jurkat cells, using ab118994 at 1/100 (Red), compared to a nonspecific negative control antibody (Blue).

References for Anti-DCK antibody [16E12] (ab118994)

ab118994 has not yet been referenced specifically in any publications.

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