Anti-DCK antibody [OTI16E12] (ab118994)
Key features and details
- Mouse monoclonal [OTI16E12] to DCK
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-DCK antibody [OTI16E12]
See all DCK primary antibodies -
Description
Mouse monoclonal [OTI16E12] to DCK -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human DCK aa 1-300. (NP_000779) produced in HEK293T cells.
Database link: P27707 -
Positive control
- WB: HEK293T cells transfected with pCMV6-ENTRY DCK. Flow Cyt (Intra): HEK-293T transfected with DCK, HeLa and Jurkat cells.
-
General notes
The clone number has been updated from 16E12 to OTI16E12, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI16E12 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB |
1/2000. Predicted molecular weight: 31 kDa.
|
Notes |
---|
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/2000. Predicted molecular weight: 31 kDa. |
Target
-
Function
Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. -
Sequence similarities
Belongs to the DCK/DGK family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 1633 Human
- Omim: 125450 Human
- SwissProt: P27707 Human
- Unigene: 709 Human
-
Alternative names
- dCK antibody
- DCK protein antibody
- DCK_HUMAN antibody
see all
Images
-
All lanes : Anti-DCK antibody [OTI16E12] (ab118994) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY DCK cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 31 kDa -
Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either DCK overexpress plasmid (Red) or empty vector control plasmid (Blue) were stained for DCK using ab118994 at 1/100 dilution.
-
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DCK using ab118994 (Red) compared to a nonspecific negative control antibody (Blue).
-
Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells using ab118994 at 1/100 (Red), compared to a nonspecific negative control antibody (Blue).
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab118994 has not yet been referenced specifically in any publications.