Purification notesPurified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted |(approximately 95%) feta bovine serum. Filtered through a 0.22 micrometer membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 31 kDa.
Use at an assay dependent dilution.
FunctionRequired for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
Sequence similaritiesBelongs to the DCK/DGK family.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Anti-DCK antibody [2243C2] (ab51517) + Immunized recombinant protein
Predicted band size : 31 kDa Observed band size : 31 kDa
References for Anti-DCK antibody [2243C2] (ab51517)
This product has been referenced in:
Neschadim A et al. Cell fate control gene therapy based on engineered variants of human deoxycytidine kinase. Mol Ther20:1002-13 (2012).
Read more (PubMed: 22273576) »
Likar Y et al. A new pyrimidine-specific reporter gene: a mutated human deoxycytidine kinase suitable for PET during treatment with acycloguanosine-based cytotoxic drugs. J Nucl Med51:1395-403 (2010).
Read more (PubMed: 20810757) »