Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
Belongs to the DCK/DGK family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Detection of DCK in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab186128 at 6 µg per reaction for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 3 minutes.
Ma YT et al. Use of a genome-wide haploid genetic screen to identify treatment predicting factors: a proof-of-principle study in pancreatic cancer. Oncotarget8:63635-63645 (2017).
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