Overview

  • Product nameAnti-DCK antibody
    See all DCK primary antibodies
  • Description
    Rabbit polyclonal to DCK
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (ATPETCLHRI YLRGRNEEQG IPLEYLEKLH YKHESWLLHR TLKTNFDYLQ) of human DCK (NP_000779).

  • Positive control
    • Fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

Target

Anti-DCK antibody images

  • Anti-DCK antibody (ab83046) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

References for Anti-DCK antibody (ab83046)

This product has been referenced in:
  • Bieghs L  et al. The effects of forodesine in murine and human multiple myeloma cells. Adv Hematol 2010:131895 (2010). WB ; Mouse . Read more (PubMed: 20981156) »

See 1 Publication for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"