Overview

  • Product name
    Anti-DCL-1 antibody
    See all DCL-1 primary antibodies
  • Description
    Rabbit polyclonal to DCL-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized peptide derived from internal region of Human DCL-1

  • Positive control
    • Human lymph node tissue HT-29 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Frozen Stock (-20C). Shelf life 12 months.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab109991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 26 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DCL-1 antibody (ab109991)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DCL-1 antibody (ab109991)
    Immunohistochemistry analysis of paraffin-embedded human lymph node tissue, using ab109991. The picture on the right is treated with the synthesized peptide.
  • Western blot - DCL-1 antibody (ab109991)
    Western blot - DCL-1 antibody (ab109991)
    All lanes : Anti-DCL-1 antibody (ab109991) at 1/500 dilution

    Lane 1 : HT-29 cells
    Lane 2 : HT-29 cells with sinthesized peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa

References

ab109991 has not yet been referenced specifically in any publications.

Publishing research using ab109991? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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