The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 63 kDa.
Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionNecessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.
Tissue specificityDetected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.
Sequence similaritiesBelongs to the DCP1 family.
Cellular localizationCytoplasm > P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.
Predicted band size : 63 kDa DCP1A antibody (ab57654) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
Flow Cytometry-Dcp1a antibody(ab57654)
Overlay histogram showing HepG2 cells stained with ab57654 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57654, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-Dcp1a antibody (ab57654)
This product has been referenced in:
Nishi K et al. Control of the localization and function of a miRNA silencing component TNRC6A by Argonaute protein. Nucleic Acids Res43:9856-73 (2015).
Read more (PubMed: 26446993) »
Blanco FF et al. Transforming growth factor ß regulates P-body formation through induction of the mRNA decay factor tristetraprolin. Mol Cell Biol34:180-95 (2014).
Read more (PubMed: 24190969) »