Recombinant
RabMAb

Anti-Dcp1a antibody [EPR13822] (ab183709)

Overview

  • Product name
    Anti-Dcp1a antibody [EPR13822]
    See all Dcp1a primary antibodies
  • Description
    Rabbit monoclonal [EPR13822] to Dcp1a
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Dcp1a aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9NPI6

  • Positive control
    • 293T, HeLa and HepG2 cell lysates; Human kidney tissue; HepG2 and HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa).
ICC/IF 1/250.

Target

  • Function
    Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.
  • Tissue specificity
    Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.
  • Sequence similarities
    Belongs to the DCP1 family.
  • Cellular localization
    Cytoplasm > P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.
  • Information by UniProt
  • Database links
  • Alternative names
    • DCP1 decapping enzyme homolog A antibody
    • Dcp1a antibody
    • DCP1A_HUMAN antibody
    • Decapping enzyme hDcp1a antibody
    • Decapping mRNA 1A antibody
    • HSA275986 antibody
    • mRNA decapping enzyme 1A antibody
    • mRNA-decapping enzyme 1A antibody
    • Nbla00360 antibody
    • Putative protein product of Nbla00360 antibody
    • Smad4 interacting transcriptional co activator antibody
    • Smad4-interacting transcriptional co-activator antibody
    • Smad4-interacting transcriptional co-activator antibody
    • SMAD4IP1 antibody
    • SMIF antibody
    • Transcription factor SMIF antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dcp1a with ab183709 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (green). Counter stained with Dapi (blue).

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Dcp1a with ab183709 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/10000 dilution + 293T cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 63 kDa
    Observed band size: 75 kDa (why is the actual band size different from the predicted?)

  • ab183709 staining Dcp1a in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Control: PBS only

  • All lanes : Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 63 kDa
    Observed band size: 75 kDa (why is the actual band size different from the predicted?)

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Dcp1a with ab183709 at 1/150 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

References

ab183709 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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