Recombinant
RabMAb

Anti-Dcp1a antibody [EPR13822] (ab183709)

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Overview

  • Product name
    Anti-Dcp1a antibody [EPR13822]
    See all Dcp1a primary antibodies
  • Description
    Rabbit monoclonal [EPR13822] to Dcp1a
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Dcp1a aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9NPI6

  • Positive control
    • 293T, HeLa and HepG2 cell lysates; Human kidney tissue; HepG2 and HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa).
ICC/IF 1/250.

Target

  • Function
    Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.
  • Tissue specificity
    Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas.
  • Sequence similarities
    Belongs to the DCP1 family.
  • Cellular localization
    Cytoplasm > P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • DCP1 decapping enzyme homolog A antibody
    • Dcp1a antibody
    • DCP1A_HUMAN antibody
    • Decapping enzyme hDcp1a antibody
    • Decapping mRNA 1A antibody
    • HSA275986 antibody
    • mRNA decapping enzyme 1A antibody
    • mRNA-decapping enzyme 1A antibody
    • Nbla00360 antibody
    • Putative protein product of Nbla00360 antibody
    • Smad4 interacting transcriptional co activator antibody
    • Smad4-interacting transcriptional co-activator antibody
    • Smad4-interacting transcriptional co-activator antibody
    • SMAD4IP1 antibody
    • SMIF antibody
    • Transcription factor SMIF antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (ab183709)
    Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (ab183709)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dcp1a with ab183709 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (green). Counter stained with Dapi (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dcp1a antibody [EPR13822] (ab183709)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dcp1a antibody [EPR13822] (ab183709)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Dcp1a with ab183709 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)
    Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)
    Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/10000 dilution + 293T cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 63 kDa
    Observed band size: 75 kDa (why is the actual band size different from the predicted?)

  • Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (ab183709)
    Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (ab183709)

    ab183709 staining Dcp1a in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Control: PBS only

  • Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)
    Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)
    All lanes : Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 63 kDa
    Observed band size: 75 kDa (why is the actual band size different from the predicted?)

  • Flow Cytometry - Anti-Dcp1a antibody [EPR13822] (ab183709)
    Flow Cytometry - Anti-Dcp1a antibody [EPR13822] (ab183709)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Dcp1a with ab183709 at 1/150 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

References

ab183709 has not yet been referenced specifically in any publications.

Publishing research using ab183709? Please let us know so that we can cite the reference in this datasheet.

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