Overview

  • Product nameAnti-Dcr 2 / Dicer 2 antibody
  • Description
    Rabbit polyclonal to Dcr 2 / Dicer 2
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Fruit fly (Drosophila melanogaster) Dcr 2/ Dicer 2.

    (Peptide available as ab19340.)

  • Positive control
    • This antibody gave a positive signal in Drosophila Embryo Nuclear Extract

Properties

Applications

Our Abpromise guarantee covers the use of ab4732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/500 - 1/1000. Predicted molecular weight: 188 kDa.

Other (lower) bands are also seen in the blot (see image). We are unsure as to the identity of these - they may be cleavage products of Dcr2, or unspecific bands. These bands are all, however, blocked with the immunising peptide.

Target

  • RelevanceRNA interference is an evolutionarily conserved gene silencing pathway in which the endonuclease, Dicer cleaves double stranded RNA into small interfering RNAs. Dicer is a multidomain protein related to the RNase III protein family. Dicer is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small interfering RNA (siRNA) component that represses gene expression. Dicer related RNA interference machinery is also involved in the formation of the heterochromatin structure in organisms such as yeast and higher vertebrate cells. In mammalian cells, both microRNAs (miRNAs) and small interfering RNAs (siRNAs) are thought to be loaded into the same RNA induced silencing complex (RISC), where they guide mRNA degradation or translation silencing depending on the complementarity of the target. Two transcript variants encoding the same protein have been identified. The Saccharomyces cerevisiae cell cycle regulator genes DCR2 and DCR1 appear to be involved in the initiation of DNA replication. It has been predicted that DCR2 might encode a protein with phosphoesterase activity.
  • Cellular localizationNuclear
  • Database links
    • Alternative names
      • Dcr2 antibody
      • Dicer antibody
      • Dicer2 antibody

    Anti-Dcr 2 / Dicer 2 antibody images

    • ab4732 staining Dcr 2 / Dicer 2 (green) in fruit fly (Drosophilia melanogaster) OSS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Tween and blocked with 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 0.2% Tween) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. ab24609, anti-Nuclear Pore Complex Proteins, was used as a nuclear membrane marker (red).

      See Abreview

    • Anti-Dcr 2 / Dicer 2 antibody (ab4732) at 1 µg/ml + Drosophila embryo nuclear extract (from D. melanogaster embryos 0-12Hr) at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 188 kDa
      Observed band size : 175,200 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 33 kDa,40 kDa,50 kDa,55 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 8 minutes
      This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab4732 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    References for Anti-Dcr 2 / Dicer 2 antibody (ab4732)

    This product has been referenced in:
    • Cernilogar FM  et al. RNA-interference components are dispensable for transcriptional silencing of the drosophila bithorax-complex. PLoS One 8:e65740 (2013). Read more (PubMed: 23785447) »
    • Philip F  et al. Phospholipase Cß1 is linked to RNA interference of specific genes through translin-associated factor X. FASEB J : (2012). Read more (PubMed: 22889834) »

    See all 5 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application IHC - Wholemount
    Sample Fruit fly (Drosophila melanogaster) Tissue (CNS)
    Specification CNS
    Username

    Miss. Rashmi Sipani

    Verified customer

    Submitted Dec 10 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample Fruit fly (Drosophila melanogaster) Cell (Oss)
    Specification Oss
    Permeabilization Yes - Tween 0,2%
    Fixative Formaldehyde
    Username

    Ms. Nathalie GReD Gueguen

    Verified customer

    Submitted Oct 24 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"