Overview

  • Product nameAnti-DcR1 antibody
    See all DcR1 primary antibodies
  • Description
    Rabbit polyclonal to DcR1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    CKEGTFRNENSPE

    , corresponding to amino acids 111-123 of Human DcR1.

  • Positive control
    • HeLa whole cell lysate.
  • General notes


    Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumour cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL (1-3). Two decoy-receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts were expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPBS with 0.02% sodium azide
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesApoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumour cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL (1-3). Two decoy-receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts were expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab2087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 65 kDa.Can be blocked with DcR1 peptide (ab6096). Can be blocked with DcR1 peptide (human).

Target

  • RelevanceDcR1 / TRAIL-R3 / TRID / LIT is one of the two putative decoy receptors identified for TRAIL, one of the members of TNF family of apoptosis inducing proteins. The other putative decoy receptor is called DcR2 / TRUNDD / TRAIL-R4. DcR1 is attached to the cell surface through glycophospholipid anchor. It has the extracellular TRAIL binding domain but lacks the cytoplasmic domain to induce apoptotic signal. Hence overexpression of DcR1 inhibits the TRAIL induced apoptosis.
  • Cellular localizationCell Membrane and Cytoplasmic
  • Database links
  • Alternative names
    • Antagonist decoy receptor for TRAIL/Apo 2L antibody
    • CD263 antibody
    • Cytotoxic TRAIL receptor 3 antibody
    • DCR1 antibody
    • DCR1 TNFR antibody
    • Decoy Receptor 1 antibody
    • Decoy TRAIL receptor without death domain antibody
    • LIT antibody
    • Lymphocyte inhibitor of TRAIL antibody
    • MGC149501 antibody
    • MGC149502 antibody
    • TNF related apoptosis inducing ligand receptor 3 antibody
    • TNF-re ated apoptosis inducing ligand receptor 3 antibody
    • TNFRSF10C antibody
    • TRAIL R3 antibody
    • TRAIL receptor 3 antibody
    • TRAILR3 antibody
    • TRID antibody
    • Tumor necrosis factor receptor superfamily member 10C antibody
    • Tumor necrosis factor receptor superfamily, member 10c, decoy without an intracellular domain antibody
    see all

Anti-DcR1 antibody images

  • All lanes : Anti-DcR1 antibody (ab2087) at 1 µg/ml

    Lane 1 : Hela cell lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Rat liver tissue lysate


    Predicted band size : 65 kDa
  • ab2087 at 10µg/ml staining DcR1 in  rat liver tissue by ICC/IF 

  • Immunofluorescence of DcR1 in Rat Liver cells using ab2087 at 20 ug/ml.

References for Anti-DcR1 antibody (ab2087)

This product has been referenced in:
  • Ziauddin MF  et al. TRAIL gene-armed oncolytic poxvirus and oxaliplatin can work synergistically against colorectal cancer. Gene Ther 17:550-9 (2010). Read more (PubMed: 20182517) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. Based on the immunogen, it looks like ab2087 will probably cross-react with DcR2. Out of the 13 residues; all have either high homology or identical residues. The antibody has not been tested for this cross-reaction. P...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"