The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: 1/2000. Detects a band of approximately 75 kDa (predicted molecular weight: 20 kDa).
We recommend using about 2.5 ug of protein in the colorimetric method, and about 10x less for ECL detection.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionSupplies the nucleotide substrate for thymidylate synthetase.
Sequence similaritiesBelongs to the cytidine and deoxycytidylate deaminase family.