Anti-DCTN1/p150-glued antibody (ab11806)
Key features and details
- Goat polyclonal to DCTN1/p150-glued
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-DCTN1/p150-glued antibody
See all DCTN1/p150-glued primary antibodies -
Description
Goat polyclonal to DCTN1/p150-glued -
Host species
Goat -
Specificity
This antibody is expected to recognise both human isoforms. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Drosophila melanogaster -
Immunogen
Synthetic peptide corresponding to Human DCTN1/p150-glued aa 1266-1278 (C terminal).
Sequence:C-QEQLHQLHSRLIS
(Peptide available asab23214) -
Positive control
- WB: HeLa cell lysates and MCF-7 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (2) |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 150 kDa.
A 1 hour primary incubation is recommended for this product. Approx 150kDa band observed in A549 and Human Testis lysates |
Notes |
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IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 150 kDa. A 1 hour primary incubation is recommended for this product. Approx 150kDa band observed in A549 and Human Testis lysates |
Target
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Function
Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. -
Tissue specificity
Brain. -
Involvement in disease
Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B) [MIM:607641]; also known as progressive lower motor neuron disease (PLMND). HMN7B is a neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
Defects in DCTN1 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
Defects in DCTN1 are the cause of Perry syndrome (PERRYS) [MIM:168605]; also called parkinsonism with alveolar hypoventilation and mental depression. Perry syndrome is a neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss. Parkinsonism develops later and respiratory failure occurred terminally. -
Sequence similarities
Belongs to the dynactin 150 kDa subunit family.
Contains 1 CAP-Gly domain. -
Post-translational
modificationsUbiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 39536 Drosophila melanogaster
- Entrez Gene: 1639 Human
- Entrez Gene: 29167 Rat
- Omim: 601143 Human
- SwissProt: P13496 Drosophila melanogaster
- SwissProt: Q14203 Human
- SwissProt: P28023 Rat
- Unigene: 12980 Drosophila melanogaster
see all -
Alternative names
- 150 kDa dynein associated polypeptide antibody
- 150 kDa dynein-associated polypeptide antibody
- DAP 150 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (14)
ab11806 has been referenced in 14 publications.
- Castellano-Pellicena I et al. Melanin Distribution in Human Skin: Influence of Cytoskeletal, Polarity, and Centrosome-Related Machinery of Stratum basale Keratinocytes. Int J Mol Sci 22:N/A (2021). PubMed: 33808676
- Deshimaru M et al. DCTN1 Binds to TDP-43 and Regulates TDP-43 Aggregation. Int J Mol Sci 22:N/A (2021). PubMed: 33924373
- Shanmugapriya S et al. Dynactin 1 negatively regulates HIV-1 infection by sequestering the host cofactor CLIP170. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34686593
- Tian WT et al. New phenotype of DCTN1-related spectrum: early-onset dHMN plus congenital foot deformity. Ann Clin Transl Neurol 7:200-209 (2020). PubMed: 32023010
- Lee M et al. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. J Cell Biol 219:N/A (2020). PubMed: 31910261