Overview

  • Product nameAnti-DCTN3 antibody
    See all DCTN3 primary antibodies
  • Description
    Goat polyclonal to DCTN3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-QVALGNISSKRE

    , corresponding to internal sequence amino acids 41-52 of Human DCTN3 (NP_009165.1).

  • Positive control
    • Human kidney lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab109551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).

Target

  • FunctionTogether with dynein may be involved in spindle assembly and cytokinesis.
  • Tissue specificityUbiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain.
  • Sequence similaritiesBelongs to the dynactin subunit 3 family.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Colocalizes with dynein to the cleavage furrow and to midbody of dividing cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • DCNT22 antibody
    • DCTN 22 antibody
    • DCTN22 antibody
    • Dctn3 antibody
    • DCTN3_HUMAN antibody
    • dynactin 3 (p22) antibody
    • dynactin 3 antibody
    • dynactin 3 isoform 1 antibody
    • Dynactin complex subunit 22 kDa subunit antibody
    • dynactin light chain antibody
    • Dynactin subunit 3 antibody
    • p22 antibody
    see all

Anti-DCTN3 antibody images

  • All lanes : Anti-DCTN3 antibody (ab109551) at 0.20000000298 µg/ml

    Lane 1 : Mouse skeletal muscle lysate
    Lane 2 : Rat skeletal muslce lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 21 kDa
  • Anti-DCTN3 antibody (ab109551) at 0.03 µg/ml + Human kidney lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 21 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

References for Anti-DCTN3 antibody (ab109551)

ab109551 has not yet been referenced specifically in any publications.

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