The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
FunctionTogether with dynein may be involved in spindle assembly and cytokinesis.
Tissue specificityUbiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain.
Sequence similaritiesBelongs to the dynactin subunit 3 family.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Colocalizes with dynein to the cleavage furrow and to midbody of dividing cells.