Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human DCTN3 aa 100-200.
293T, HeLa, BxPC 3, SH-SY5Y and Mouse and Rat muscle lysates; Human breast tissue.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
FunctionTogether with dynein may be involved in spindle assembly and cytokinesis.
Tissue specificityUbiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain.
Sequence similaritiesBelongs to the dynactin subunit 3 family.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Colocalizes with dynein to the cleavage furrow and to midbody of dividing cells.
Immunohistochemical staining of paraffin embedded human pancreas with purified ab124674 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunofluorescence staining of BxPC-3 cells with purified ab124674 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab124674 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab15120) at a dilution of 1/500.
Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
All lanes : Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysates Lane 2 : Mouse muscle lysates Lane 3 : HeLa cell lysates Lane 4 : BxPC 3 cell lysates Lane 5 : SH-SY5Y cell lysates Lane 6 : Rat muscle lysates