Overview

  • Product nameAnti-DCTN3 antibody [EPR5097]
    See all DCTN3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5097] to DCTN3
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human DCTN3 aa 100-200.

  • Positive control
    • 293T, HeLa, BxPC 3, SH-SY5Y and Mouse and Rat muscle lysates; Human breast tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab124674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/2000 - 1/10000.
WB 1/2000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

Target

  • FunctionTogether with dynein may be involved in spindle assembly and cytokinesis.
  • Tissue specificityUbiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain.
  • Sequence similaritiesBelongs to the dynactin subunit 3 family.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Midbody. Localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Colocalizes with dynein to the cleavage furrow and to midbody of dividing cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • DCNT22 antibody
    • DCTN 22 antibody
    • DCTN22 antibody
    • Dctn3 antibody
    • DCTN3_HUMAN antibody
    • dynactin 3 (p22) antibody
    • dynactin 3 antibody
    • dynactin 3 isoform 1 antibody
    • Dynactin complex subunit 22 kDa subunit antibody
    • dynactin light chain antibody
    • Dynactin subunit 3 antibody
    • p22 antibody
    see all

Anti-DCTN3 antibody [EPR5097] images

  • All lanes : Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/10000 dilution (purified)

    Lane 1 : 293T cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 21 kDa
    Observed band size : 21 kDa

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/10000 dilution (purified) + Rat muscle at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 21 kDa
    Observed band size : 21 kDa

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/2000 dilution (purified) + Mouse muscle at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 21 kDa
    Observed band size : 21 kDa

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human pancreas with purified ab124674 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunofluorescence staining of BxPC-3 cells with purified ab124674 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab124674 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab15120) at a dilution of 1/500.

  • All lanes : Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/1000 dilution (unpurified)

    Lane 1 : 293T cell lysates
    Lane 2 : Mouse muscle lysates
    Lane 3 : HeLa cell lysates
    Lane 4 : BxPC 3 cell lysates
    Lane 5 : SH-SY5Y cell lysates
    Lane 6 : Rat muscle lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1, 2, 3, 5 & 6 : Goat anti-Rabbit HRP at 1/2000 dilution


    Predicted band size : 21 kDa
  • Immunohistochemical staining of DCTN3 in paraffin embedded human breast tissue by unpurified ab124674 at 1/100 dilution.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-DCTN3 antibody [EPR5097] (ab124674)

ab124674 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab124674.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"