Recombinant
RabMAb

Anti-DCTN5 antibody [EPR11253] (ab157197)

Overview

  • Product name
    Anti-DCTN5 antibody [EPR11253]
    See all DCTN5 primary antibodies
  • Description
    Rabbit monoclonal [EPR11253] to DCTN5
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human DCTN5.

  • Positive control
    • MCF7, Human fetal liver, and SH-SY5Y lysates; Human kidney tissue; MCF7 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab157197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    Images

    • All lanes : Anti-DCTN5 antibody [EPR11253] (ab157197) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Human fetal liver cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 20 kDa
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCTN5 with ab157197 at 1/100 dilution.
    • Immunofluorescent analysis of MCF7 cells labeling DCTN5 with ab157197 at 1/250 dilution.
    • Detection of DCTN5 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab156197 at 1/10 dilution.

    References

    ab157197 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African green monkey Cell (Kidney)
    Permeabilization
    Yes - 0.2% triton x 100
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
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    Submitted May 18 2017

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3% triton x100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative
    Paraformaldehyde
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    Submitted Apr 13 2017

    Application
    Western blot
    Sample
    Cow Cell lysate - whole cell (Lung)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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    Submitted Apr 05 2017

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-12)
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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    Submitted Apr 05 2017

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Brain)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-12)
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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    Submitted Apr 05 2017

    Application
    Western blot
    Sample
    African green monkey Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Apr 05 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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