Overview

  • Product nameAnti-DCUN1D1 antibody
    See all DCUN1D1 primary antibodies
  • Description
    Goat polyclonal to DCUN1D1
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide:

    C-RPQIAGTKSTT

    , corresponding to C terminal amino acids 248-258 of Human DCUN1D1 (NP_065691.2).

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 30 kDa.

Target

  • RelevanceDCUN1D1 (DCUN1 domain-containing protein 1) contains a DUF298 domain and a UBA-like ubiquitin. Forms part of an E3 ubiquitin ligase complex for neddylation. Required for neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes by enhancing the rate of cullins neddylation. Functions to recruit the NEDD8-charged E2 enzyme to the cullin component. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic progression. Defects in DCUN1D1 may be a cause of squamous cell carcinomas. Strongly overexpressed in thyroid tumors, bronchioloalveolar carcinomas, and malignant tissues of squamous cell carcinoma of the oral tongue. Not overexpressed in aggressive adrenocortical carcinomas.
  • Database links
  • Alternative names
    • DCN1 defective in cullin neddylation 1 domain containing 1 antibody
    • DCN1 like protein 1 antibody
    • DCNL1 antibody
    • DCUN1 domain containing protein 1 antibody
    • DCUN1L1 antibody
    • Defective in cullin neddylation protein 1 like protein 1 antibody
    • RP 42 antibody
    • RP42 antibody
    • RP42 homolog antibody
    • SCCRO antibody
    • SCRO antibody
    • Squamous cell carcinoma related oncogene antibody
    • Tes 3 antibody
    • Tes3 antibody
    see all

Anti-DCUN1D1 antibody images

  • ab99506 (5µg/ml) staining of paraffin embedded Human Kidney shows pixulated staining in the cytoplasm of epithelial cells of PCT, but heavy cytoplasm staining in the epithelial cells of the DCT. Tissue treated by steamed antigen retrieval with citrate buffer pH 6, AP-staining.

  • Anti-DCUN1D1 antibody (ab99506) at 0.5 µg/ml + HepG2 cell lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 30 kDa


    Exposure time : 1 hour

References for Anti-DCUN1D1 antibody (ab99506)

ab99506 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"