Recombinant
RabMAb

Anti-DCUN1D1 antibody [EPR13492] (ab181233)

Overview

  • Product name
    Anti-DCUN1D1 antibody [EPR13492]
    See all DCUN1D1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13492] to DCUN1D1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human DCUN1D1 aa 50-200. The exact sequence is proprietary.
    Database link: Q96GG9

  • Positive control
    • A549, HT29, HepG2 and HeLa cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab181233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 30 kDa.
IP 1/30 - 1/50.

Target

  • Relevance
    DCUN1D1 (DCUN1 domain-containing protein 1) contains a DUF298 domain and a UBA-like ubiquitin. Forms part of an E3 ubiquitin ligase complex for neddylation. Required for neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes by enhancing the rate of cullins neddylation. Functions to recruit the NEDD8-charged E2 enzyme to the cullin component. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic progression. Defects in DCUN1D1 may be a cause of squamous cell carcinomas. Strongly overexpressed in thyroid tumors, bronchioloalveolar carcinomas, and malignant tissues of squamous cell carcinoma of the oral tongue. Not overexpressed in aggressive adrenocortical carcinomas.
  • Database links
  • Alternative names
    • DCN1 defective in cullin neddylation 1 domain containing 1 antibody
    • DCN1 like protein 1 antibody
    • DCNL1 antibody
    • DCUN1 domain containing protein 1 antibody
    • DCUN1L1 antibody
    • Defective in cullin neddylation protein 1 like protein 1 antibody
    • RP 42 antibody
    • RP42 antibody
    • RP42 homolog antibody
    • SCCRO antibody
    • SCRO antibody
    • Squamous cell carcinoma related oncogene antibody
    • Tes 3 antibody
    • Tes3 antibody
    see all

Images

  • All lanes : Anti-DCUN1D1 antibody [EPR13492] (ab181233) at 1/20000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : HT29 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 30 kDa
  • Immunoprecipitation analysis of HeLa cells labeling DCUN1D1 using ab181233 at 1/50 dilution. A Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at a 1/1500 dilution.

References

ab181233 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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