Overview

  • Product nameAnti-DCUN1D3 antibody
    See all DCUN1D3 primary antibodies
  • Description
    Rabbit polyclonal to DCUN1D3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (HREEQVPPCG KPGGDILVNG TKKAEAATEA CQLPTSSGDA GRESKSNAEE) of Human DCUN1D3 (NP_775746).

  • Positive control
    • Fetal kidney lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionAntagonizes DCUN1D1-mediated CUL1 neddylation by sequestering CUL1 at the cell membrane (PubMed:25349211). When overexpressed in transformed cells, may promote mesenchymal to epithelial-like changes and inhibit colony formation in soft agar (PubMed:25349211).
  • Tissue specificityTends to be down-regulated in different type of cancers, including lung neuroendocrine carcinoma, thyroid Huerthle cell carcinoma and lung squamous cell carcinoma.
  • Sequence similaritiesContains 1 DCUN1 domain.
  • DomainThe DCUN1 domain, also known as PONY domain, mediates recognition of the N-terminally acetylated NEDD8-conjugating E2 enzyme. This domain is also involved in CAND1-, CUL1-, CUL3- and RBX1-binding.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 44M2.4 antibody
    • DCN1 defective in cullin neddylation 1 domain containing 3 (S. cerevisiae) antibody
    • DCN1 defective in cullin neddylation 1 domain containing 3 antibody
    • DCN1 like protein 3 antibody
    • DCN1-like protein 3 antibody
    • DCNL3_HUMAN antibody
    • DCUN1 domain containing protein 3 antibody
    • DCUN1 domain-containing protein 3 antibody
    • dcun1d3 antibody
    • Defective in cullin neddylation protein 1 like protein 3 antibody
    • Defective in cullin neddylation protein 1-like protein 3 antibody
    • DKFZp686O0290 antibody
    • FLJ41725 antibody
    • MGC48972 antibody
    • Squamous cell carcinoma-related oncogene 3 antibody
    see all

Anti-DCUN1D3 antibody images

  • Anti-DCUN1D3 antibody (ab94454) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal kidney lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa

References for Anti-DCUN1D3 antibody (ab94454)

ab94454 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"