The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionAntagonizes DCUN1D1-mediated CUL1 neddylation by sequestering CUL1 at the cell membrane (PubMed:25349211). When overexpressed in transformed cells, may promote mesenchymal to epithelial-like changes and inhibit colony formation in soft agar (PubMed:25349211).
Tissue specificityTends to be down-regulated in different type of cancers, including lung neuroendocrine carcinoma, thyroid Huerthle cell carcinoma and lung squamous cell carcinoma.
Sequence similaritiesContains 1 DCUN1 domain.
DomainThe DCUN1 domain, also known as PONY domain, mediates recognition of the N-terminally acetylated NEDD8-conjugating E2 enzyme. This domain is also involved in CAND1-, CUL1-, CUL3- and RBX1-binding.