Overview

  • Product name
    Anti-DCV capsid polyprotein antibody
  • Description
    Rabbit polyclonal to DCV capsid polyprotein
  • Tested applications
    Suitable for: WB, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Drosophila C virus
  • Immunogen

    Synthetic peptide corresponding to C terminal residues of Drosophila C virus DCV capsid polyprotein.

Properties

Applications

Our Abpromise guarantee covers the use of ab92954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 100 kDa). PubMed: 26546607

The predicted MW of the capsid polyprotein is around 100kDa. But, after infection of DCV to cells, the polyprotein is cleaved into several fragments with around 30kDa by proteases (shown in Nat. Immunol. 2004 5:81).

ELISA 1/2000 - 1/5000.
ICC Use at an assay dependent concentration. PubMed: 26546607

Target

  • Relevance
    DCV capsid polyprotein (DCVgp2) includes rhv_like or Picornavirus capsid protein domain_like and CRPV_capsid domains. Picornaviruses are non-enveloped plus-strand ssRNA animal viruses with icosahedral capsids composed of 60 copies each of 4 virus encoded proteins. Alignment includes picornaviridae, like poliovirus, hepatitis A virus, rhinovirus, foot-and-mouth disease virus and encephalomyocarditis virus. The common structure of DCV capsid polyprotein is an 8-stranded beta sandwich.
  • Database links
    • Alternative names
      • DCVgp2 antibody
      • DCVgp2 capsid polyprotein antibody

    References for Anti-DCV capsid polyprotein antibody (ab92954)

    This product has been referenced in:
    • Nainu F  et al. Protection of Insects against Viral Infection by Apoptosis-Dependent Phagocytosis. J Immunol N/A:N/A (2015). WB, ICC ; Drosophila C virus . Read more (PubMed: 26546607) »

    See 1 Publication for this product

    Product Wall

    Application
    Western blot
    Loading amount
    500000 cells
    Gel Running Conditions
    Non-reduced Denaturing (12 %)
    Sample
    Drosophila C Virus Cell lysate - whole cell (Drosophila S2 cell)
    Specification
    Drosophila S2 cell
    Treatment
    DCV MOI=0,1 0,01 or 0,001 for 2hrs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Ms. Barbara Viginier

    Verified customer

    Submitted Jan 22 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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