This antibody gave a positive signal in both HEK293 and HepG2 whole cell lysates as well as the following tissue lysates: Human Fetal Liver; Human Liver; Human Kidney; Human Pancreas.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
NG NG dimethylarginine dimethylaminohydrolase antibody
Western blot - Anti-DDAH1 antibody (ab127579)
All lanes : Anti-DDAH1 antibody (ab127579) at 1 µg/ml
Lane 1 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282) Lane 2 : Human liver tissue lysate - total protein (ab29889) Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : Human kidney tissue lysate - total protein (ab30203) Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 6 : Human pancreas tissue lysate - total protein (ab29816)
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
IHC image of DDAH1 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab127579, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.