Overview

  • Product name
  • Description
    Rabbit polyclonal to DDAH1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human DDAH1 aa 235-285 (C terminal). The exact sequence is proprietary.
    Sequence:

    LHRTPE EYPESAKVYE KLKDHMLIPV SMSELEKVDG LLTCCSVLIN KKVDS


    Database link: O94760

  • Positive control
    • 293T whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab217063 was affinity purified using an epitope specific to DDAH1 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab217063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
    • Tissue specificity
      Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
    • Sequence similarities
      Belongs to the DDAH family.
    • Information by UniProt
    • Database links
    • Alternative names
      • DDAH antibody
      • DDAH I antibody
      • DDAH-1 antibody
      • DDAH1 antibody
      • DDAH1_HUMAN antibody
      • DDAHI antibody
      • Dimethylargininase 1 antibody
      • Dimethylargininase-1 antibody
      • Dimethylarginine dimethylaminohydrolase 1 antibody
      • N(G) antibody
      • N(G)-dimethylarginine dimethylaminohydrolase 1 antibody
      • NG NG dimethylarginine dimethylaminohydrolase antibody
      see all

    Images

    • Detection of DDAH1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab217063 at 6 µg/mg lysate for IP (Lane 1). For WB detection anti-DDAH1 antibody was used at 1µg/ml. Lane 2 Control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    ab217063 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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