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Rabbit polyclonal to DDAH1
Predicted to work with:
Synthetic peptide within Human DDAH1 aa 235-285 (C terminal). The exact sequence is proprietary.
LHRTPE EYPESAKVYE KLKDHMLIPV SMSELEKVDG LLTCCSVLIN KKVDS
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate pH 7-8
Concentration information loading...
Immunogen affinity purified
ab217063 was affinity purified using an epitope specific to DDAH1 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Is unsuitable for WB.
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
Belongs to the DDAH family.
Information by UniProt
DDAH I antibody
Immunoprecipitation - Anti-DDAH1 antibody (ab217063)
Detection of DDAH1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab217063 at 6 µg/mg lysate for IP (Lane 1). For WB detection anti-DDAH1 antibody was used at 1µg/ml. Lane 2 Control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
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