Overview

  • Product name
  • Description
    Goat polyclonal to DDAH1
  • Tested applications
    Suitable for: IHC-P, ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:

    TCCSVLINKKVDS

    , corresponding to C terminal amino acids 272-284 of Human DDAH1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 24663083
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).Can be blocked with Human DDAH1 peptide (ab99047).

Target

  • Function
    Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
  • Tissue specificity
    Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
  • Sequence similarities
    Belongs to the DDAH family.
  • Information by UniProt
  • Database links
  • Alternative names
    • DDAH antibody
    • DDAH I antibody
    • DDAH-1 antibody
    • DDAH1 antibody
    • DDAH1_HUMAN antibody
    • DDAHI antibody
    • Dimethylargininase 1 antibody
    • Dimethylargininase-1 antibody
    • Dimethylarginine dimethylaminohydrolase 1 antibody
    • N(G) antibody
    • N(G)-dimethylarginine dimethylaminohydrolase 1 antibody
    • NG NG dimethylarginine dimethylaminohydrolase antibody
    see all

Anti-DDAH1 antibody images

  • Anti-DDAH1 antibody (ab2231) at 0.10000000149 µg/ml + Human kidney lysate at 35 µg

    Predicted band size : 31 kDa
  • ICC/IF image of ab2231 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2231, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab2231 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2231, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-DDAH1 antibody (ab2231)

This product has been referenced in:
  • Jung CS  et al. Endogenous nitric-oxide synthase inhibitor ADMA after acute brain injury. Int J Mol Sci 15:4088-103 (2014). IHC-Fr ; Rat . Read more (PubMed: 24663083) »
  • Ghebremariam YT  et al. FXR agonist INT-747 upregulates DDAH expression and enhances insulin sensitivity in high-salt fed Dahl rats. PLoS One 8:e60653 (2013). WB ; Rat . Read more (PubMed: 23593273) »

See all 7 Publications for this product

Product Wall

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain (Hippocampus))
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Brain (Hippocampus)
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

圭一 角山

Verified customer

Submitted Nov 25 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (hippocampus)
Loading amount
5 µg
Specification
hippocampus
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Hippocampus lysate)
Loading amount
40 µg
Specification
Hippocampus lysate
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Richard D'Mello

Verified customer

Submitted Apr 14 2009

According to the datasheet, this antibody is thought to run at 40kDa due glycosylation. It could be that the protein is running at 32kDa in their experiments as the protein in their system is not glycosylated. However, I am concerned that their negativ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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