Recombinant Anti-DDAH1 antibody [EPR13921] (ab181859)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13921] to DDAH1
- Suitable for: Flow Cyt (Intra), IHC-P, WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DDAH1 antibody [EPR13921]
See all DDAH1 primary antibodies -
Description
Rabbit monoclonal [EPR13921] to DDAH1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431, HuVEC, HepG2 and 293 cell lysates. IP: 293 cells. Flow Cyt (intra): 293 cells. IHC-P: Human hepatocellular carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13921 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa).
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IP |
1/40.
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Notes |
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Flow Cyt (Intra)
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa). |
IP
1/40. |
Target
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Function
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. -
Tissue specificity
Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle. -
Sequence similarities
Belongs to the DDAH family. - Information by UniProt
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Database links
- Entrez Gene: 23576 Human
- Omim: 604743 Human
- SwissProt: O94760 Human
- Unigene: 379858 Human
- Unigene: 713411 Human
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Alternative names
- DDAH antibody
- DDAH I antibody
- DDAH-1 antibody
see all
Images
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All lanes : Anti-DDAH1 antibody [EPR13921] (ab181859) at 1/10000 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : DDAH1 knockout A431 cell lysate
Lane 3 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 4 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab181859 observed at 37 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab181859 was shown to react with DDAH1 in wild-type A431 cells in Western blot. Loss of signal was observed when DDAH1 knockout sample was used. Wild-type A431 and DDAH1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab181859 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-DDAH1 antibody [EPR13921] (ab181859) at 1/5000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 31 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
Western blot analysis of 293 cell lysate immunoprecipitated using ab181859 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody was used at 1/1000 dilution.
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling DDAH1 with ab181859 at 1/30 dilution (red), compared to a Rabbit monoclonal IgGisotype control (green), followed by Goat anti-Rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling DDAH1 with ab181859 at 1/50 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181859 has not yet been referenced specifically in any publications.