Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab1383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).Can be blocked with Human DDAH2 peptide (ab22866). Please note that DDAH2, according to NP_039268, has a consensus glycosylation site. Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].
IHC-FoFr Use at an assay dependent dilution. PubMed: 21408057
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionHydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
  • Tissue specificityDetected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain.
  • Sequence similaritiesBelongs to the DDAH family.
  • Information by UniProt
  • Database links
  • Alternative names
    • DDAH antibody
    • DDAH II antibody
    • DDAH-2 antibody
    • DDAH2 antibody
    • DDAH2_HUMAN antibody
    • DDAHII antibody
    • Dimethylargininase 2 antibody
    • Dimethylargininase-2 antibody
    • Dimethylarginine dimethylaminohydrolase 2 antibody
    • Dimethylarginine dimethylaminohydrolase II antibody
    • G6a antibody
    • N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 antibody
    • NG dimethylarginine dimethylamino hydrolase homolog antibody
    • NG30 antibody
    • OTTHUMP00000029307 antibody
    • OTTHUMP00000029310 antibody
    • OTTHUMP00000174488 antibody
    • OTTHUMP00000174489 antibody
    • Protein G6a antibody
    • S phase protein antibody
    • S-phase protein antibody
    see all

Anti-DDAH2 antibody images

  • Anti-DDAH2 antibody (ab1383) at 1 µg/ml + Human Heart extracts at 35 µg

    Predicted band size : 30 kDa

References for Anti-DDAH2 antibody (ab1383)

This product has been referenced in:
  • Jung CS  et al. Endogenous nitric-oxide synthase inhibitor ADMA after acute brain injury. Int J Mol Sci 15:4088-103 (2014). IHC-Fr ; Rat . Read more (PubMed: 24663083) »
  • Shao Z  et al. Pulmonary hypertension associated with advanced systolic heart failure: dysregulated arginine metabolism and importance of compensatory dimethylarginine dimethylaminohydrolase-1. J Am Coll Cardiol 59:1150-8 (2012). WB ; Human . Read more (PubMed: 22440215) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Hippocampus)
Loading amount 40 µg
Specification Hippocampus
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Richard D'Mello

Verified customer

Submitted Apr 14 2009

Thank you for your enquiry. This is a well sold product and has been around for quite a while and we are currently selling the third batch. Interestingly, the very first batch did react with a 40kDa band in lysates of Mouse Heart. This would be cons...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"