Overview

  • Product name
  • Description
    Rabbit polyclonal to DDAH2
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (GTPGEGLGRC SHALIRGVPE SLASGEGAGA GLPALDLAKA QREHGVLGGK) of Human DDAH2 (NP_039268).

  • Positive control
    • Human fetal brain lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
  • Tissue specificity
    Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain.
  • Sequence similarities
    Belongs to the DDAH family.
  • Information by UniProt
  • Database links
  • Alternative names
    • DDAH antibody
    • DDAH II antibody
    • DDAH-2 antibody
    • DDAH2 antibody
    • DDAH2_HUMAN antibody
    • DDAHII antibody
    • Dimethylargininase 2 antibody
    • Dimethylargininase-2 antibody
    • Dimethylarginine dimethylaminohydrolase 2 antibody
    • Dimethylarginine dimethylaminohydrolase II antibody
    • G6a antibody
    • N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 antibody
    • NG dimethylarginine dimethylamino hydrolase homolog antibody
    • NG30 antibody
    • OTTHUMP00000029307 antibody
    • OTTHUMP00000029310 antibody
    • OTTHUMP00000174488 antibody
    • OTTHUMP00000174489 antibody
    • Protein G6a antibody
    • S phase protein antibody
    • S-phase protein antibody
    see all

Images

  • Anti-DDAH2 antibody (ab87064) at 1 µg/ml (5% skim milk / PBS buffer ) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • ab87064 at 5 µg/ml staining DDAH2 in Human heart tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab87064 at 5 µg/ml staining DDAH2 in Human uterus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

ab87064 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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