Overview

  • Product nameAnti-DDB1 antibody [EPR6089]
    See all DDB1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6089] to DDB1
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues at the C-terminus of Human DDB1.

  • Positive control
    • HepG2, HeLa, NIH3T3, and Human platelet lysates, Human breast tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: UV-treated HeLa
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-DDB1 antibody (HRP) [EPR6089] (ab199026)

Properties

Applications

Our Abpromise guarantee covers the use of ab109027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/50000 - 1/200000. Detects a band of approximately 130 kDa (predicted molecular weight: 127 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionRequired for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the DDB1 family.
    • Post-translational
      modifications
      Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.
    • Cellular localizationCytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
    • Information by UniProt
    • Database links
    • Alternative names
      • Damage specific DNA binding protein 1 antibody
      • Damage-specific DNA-binding protein 1 antibody
      • DDB 1 antibody
      • DDB p127 subunit antibody
      • Ddb1 antibody
      • DDB1_HUMAN antibody
      • DDBa antibody
      • DNA damage binding protein 1 antibody
      • DNA damage-binding protein 1 antibody
      • DNA damage-binding protein a antibody
      • HBV X-associated protein 1 antibody
      • UV damaged DNA binding factor antibody
      • UV damaged DNA binding protein 1 antibody
      • UV DDB 1 antibody
      • UV DDB1 antibody
      • UV-damaged DNA-binding factor antibody
      • UV-damaged DNA-binding protein 1 antibody
      • UV-DDB 1 antibody
      • X associated protein 1 antibody
      • XAP 1 antibody
      • XAP-1 antibody
      • XAP1 antibody
      • Xeroderma pigmentosum group E complementing protein antibody
      • Xeroderma pigmentosum group E-complementing protein antibody
      • XPCe antibody
      • XPE antibody
      • XPE BF antibody
      • XPE binding factor antibody
      • XPE-BF antibody
      • XPE-binding factor antibody
      see all

    Anti-DDB1 antibody [EPR6089] images

    • ab109027 stained UV-treated HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109027 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150081 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • All lanes : Anti-DDB1 antibody [EPR6089] (ab109027) at 1/50000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : NIH3T3 cell lysate
      Lane 4 : Human platelet lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 127 kDa
    • Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109027 at a dilution of 1/100.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-DDB1 antibody [EPR6089] (ab109027)

    This product has been referenced in:
    • Yu C  et al. CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation. Nat Commun 6:8017 (2015). WB ; Mouse . Read more (PubMed: 26281983) »

    See 1 Publication for this product

    Product Wall

    Merci de votre intérêt pour ab21080 et merci pour m'avoir fourni la séquence de la DDB1 du xénope.

    Nous avons à notre catalogue deux anticorps anti-DDB1 testés en Western Blot et en IP; le polyclonal de...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"