Anti-DDB1 with anti-GAPDH internal loading control antibody (ab75393)

Overview

  • Product nameAnti-DDB1 with anti-GAPDH internal loading control antibody
  • Description
    Rabbit polyclonal to DDB1 with anti-GAPDH internal loading control
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ChIP,Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Human DDB1 with anti-GAPDH internal loading control.

  • Positive control
    • This cocktail gave a positive signal in the following whole cell lysates: HeLa; HeLa Irradiated; MCF7; T47D; HepG2; Jurkat; Caco2.

Properties

Applications

Our Abpromise guarantee covers the use of ab75393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37, 127 kDa (predicted molecular weight: 37, 127 kDa). ab75393 contains 2 antibodies, generated by raising against an DDB1 immunogen and spiking with anti-GAPDH loading control.
  • Application notesIs unsuitable for ChIP,Flow Cyt,ICC or IHC-P.
  • Target

    • FunctionRequired for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the DDB1 family.
    • Post-translational
      modifications
      Phosphorylated by ABL1.
      Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.
    • Cellular localizationCytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
    • Information by UniProt
    • Database links
    • Alternative names
      • Damage-specific DNA-binding protein 1 antibody
      • DDB p127 subunit antibody
      • Ddb1 antibody
      • DDB1_HUMAN antibody
      • DDBa antibody
      • DNA damage-binding protein 1 antibody
      • DNA damage-binding protein a antibody
      • HBV X-associated protein 1 antibody
      • UV-damaged DNA-binding factor antibody
      • UV-damaged DNA-binding protein 1 antibody
      • UV-DDB 1 antibody
      • XAP-1 antibody
      • Xeroderma pigmentosum group E-complementing protein antibody
      • XPCe antibody
      • XPE-BF antibody
      • XPE-binding factor antibody
      see all

    Anti-DDB1 with anti-GAPDH internal loading control antibody images

    • All lanes : Anti-DDB1 with anti-GAPDH internal loading control antibody (ab75393) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Hela Whole Cell Lysate - UV Irradiated
      Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 4 : T47D whole cell lysate (ab14899)
      Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 7 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 37, 127 kDa
      Observed band size : 37,127 kDa
      Additional bands at : 32 kDa,60 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds

    References for Anti-DDB1 with anti-GAPDH internal loading control antibody (ab75393)

    ab75393 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"