Recombinant
RabMAb

Anti-DDB2 antibody [EPR9811] (ab181136)

Overview

  • Product name
    Anti-DDB2 antibody [EPR9811]
    See all DDB2 primary antibodies
  • Description
    Rabbit monoclonal [EPR9811] to DDB2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DDB2 aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: Q92466

  • Positive control
    • HeLa and Raji cell lysates; Human breast and cervical carcinoma tissues; HeLa cells; K562 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR9811
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Required for DNA repair. Binds to DDB1 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair.
  • Tissue specificity
    Ubiquitously expressed; with highest levels in corneal endothelium and lowest levels in brain. Isoform D1 is highly expressed in brain and heart. Isoform D2, isoform D3 and isoform D4 are weakly expressed.
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Defects in DDB2 are a cause of xeroderma pigmentosum complementation group E (XP-E) [MIM:278740]; also known as xeroderma pigmentosum V (XP5). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities.
  • Sequence similarities
    Belongs to the WD repeat DDB2/WDR76 family.
    Contains 5 WD repeats.
  • Domain
    The DWD box is required for interaction with DDB1.
  • Post-translational
    modifications
    Phosphorylation by ABL1 negatively regulate UV-DDB activity.
    Ubiquitinated by CUL4A in response to UV irradiation. Ubiquitination appears to both impair DNA-binding and promotes ubiquitin-dependent proteolysis. Degradation of DDB2 at sites of DNA damage may be a prerequisite for their recognition by XPC and subsequent repair. CUL4A-mediated degradation appears to be promoted by ABL1.
  • Cellular localization
    Nucleus. Accumulates at sites of DNA damage following UV irradiation.
  • Information by UniProt
  • Database links
  • Alternative names
    • damage-specific DNA binding protein 2 antibody
    • Damage-specific DNA-binding protein 2 antibody
    • DDB p48 subunit antibody
    • Ddb2 antibody
    • DDB2_HUMAN antibody
    • DDBb antibody
    • DNA damage-binding protein 2 antibody
    • UV-damaged DNA-binding protein 2 antibody
    • UV-DDB 2 antibody
    • Xeroderma pigmentosum group E protei antibody
    see all

Images

  • All lanes : Anti-DDB2 antibody [EPR9811] (ab181136) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 48 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DDB2 with ab181136 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling DDB2 with ab181136 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunofluorescent analysis of acetone-fixed HeLa cells labeling DDB2 with ab181136 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (left panel). Counter stained with Dapi (right panel).

  • Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling DDB2 with ab181136 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at 1/150 dilution.

References

ab181136 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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