xxxDDDDKconjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.
Our Abpromise guarantee covers the use of ab1162 in the following tested applications.
|WB||Use a concentration of 0.2 - 1 µg/ml.
Antibody was analyzed by western blot using various amino-terminal and carboxy-terminal DDDDK fusion proteins and Invitrogen Positope" R900-40. A 1/5000 dilution gave bands against 0.1, 1.0 and 10 ng of the fusion proteins and 100 ng and 25 ng of the Positope.
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||Use a concentration of 1 - 5 µg/ml.
As coating antibody in sELISA.
|IP||1/40. PubMed: 25419851|