General notesEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/20000.
1/2000 - 1/10000.
Use at an assay dependent concentration.
RelevanceThis is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
DDDDK epitope tag antibody
DYKDDDDK epitope tag antibody
DYKDDDDK tag antibody
ECS epitope tag antibody
ECS tag antibody
Enterokinase Cleavage Site epitope tag antibody
Enterokinase Cleavage Site tag antibody
FLAG tag antibody
Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) images
Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag antibody (ab1170)This image is courtesy of an anonymous Abreview
ab1170 staining DDDDK in Mouse primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 1x PBS + 5% Goat serum + 0.1% Triton-X 100) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-chicken IgY polyclonal (1/400) was used as the secondary antibody.