Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab1170)

Overview

  • Product nameAnti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma)
    See all DDDDK tag primary antibodies
  • Description
    Chicken polyclonal to DDDDK tag - (Equivalent to FLAG antibodies from Sigma)
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Immunogen

    Peptide

    xxxDDDDK

    conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.

  • General notesEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were affinity purified using the peptide coupled to a different carrier.
  • ClonalityPolyclonal
  • IsotypeIgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/20000.
WB 1/2000 - 1/10000.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceThis is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) images

  • ab1170 staining DDDDK in Mouse primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 1x PBS + 5% Goat serum + 0.1% Triton-X 100) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-chicken IgY polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab1170) at 1/5000 dilution

    Secondary
    Rabbit Anti-Chicken IgY H&L (HRP) (ab6753) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 66 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab1170)

ab1170 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Cell (Primary hippocampal neurons)
Specification Primary hippocampal neurons
Permeabilization No
Fixative Paraformaldehyde
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Verified customer

Submitted Oct 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Recombinant protein (BL21)
Loading amount 0.1 µg
Specification BL21
Gel Running Conditions Reduced Denaturing (4~20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Sep 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (Mach1cell)
Loading amount 0.5 µg
Specification Mach1cell
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Sep 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"