Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab122902)

Overview

  • Product name
    Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma)
    See all DDDDK tag primary antibodies
  • Description
    Rabbit polyclonal to DDDDK tag - (Equivalent to FLAG antibodies from Sigma)
  • Specificity
    ab12902 recognizes Flag-tagged fusion proteins.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    DYKDDDDK

  • Positive control
    • Lysate of 293T cells transfected with the plasmid carrying the Flag-tagged PRMT6 gene.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: 99% Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
  • Alternative names
    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG antibody
    • FLAG tag antibody
    see all

Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) images

  • All lanes : Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab122902) at 1/2000 dilution

    Lane 1 : Lysate of 293T cells transfected with an empty vector
    Lane 2 : Lysate of 293T cells transfected with the plasmid carrying the Flag-tagged PRMT6 gene

References for Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab122902)

ab122902 has not yet been referenced specifically in any publications.

Product Wall

This is a good question and difficult to answer. Antibodies to fusion protein tags usually detect without problems no matter where the tag is. However, you would be less likely to encounter any problems if the tag is on the N or C terminus rather than ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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